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Antigone-Analysis of Creon#8217;s speech. After the war between Oedipus two sons Eteocles and Polyneices killed both brothers, Creon, their uncle, ascended to simile crucible the throne as the nearest kinsman. This speech is Creons first as king and its main aims are to american explain his legitimacy, outline his political ideals and justify his proclamation regarding the treatment of Polyneices. Being an astute speaker, Creons speech contained effective usage of the art of persuasion, showing his shrewdness, inflexibility and arrogance. We will write a custom essay sample. on Antigone-Analysis of Creon#8217;s speech or any similar. topic specifically for simile crucible you. By contrasting Antigones earlier decision, it plays in important role in avoidable, plot development, bringing out the central theme of simile conflict, creating tension and building up the rising action. Regarding the overall structure of Creons speech, the order of his choice of topics masks his rash decision under a justified appearance, reflecting the great care taken into detailed planning.

He starts off with a conventional opening, crediting the gods dutifully for their continuous guardianship of Thebes with the commonly used ship-of-state metaphor, Gentlemen, after tossing the life of Crimes Internet Essay our city on crucible, the great waves of the ocean, the gods have safely righted it once more. This displays Creons loyalty to the gods as any good king would, presenting an appealing image of himself. The traditional element is pleasing to most conservative elders, providing them with a sense of security under the Comparsion Frost´s Stopping by Woods in a Snowy of a by Frost, new kings rule, and hence, his later judgments, however unusual it may be. Creon intentionally did not start by announcing his proclamation at the beginning as its unconventional nature tends to be frowned upon to say the least, or even be rejected by the conservative elders. Creon is very well aware of this and delays his announcement, addressing the conventional first. Afterwards, Creon moves onto flattering the simile in the, chorus (elders), recounting their faithful service under King Laius, Oedipus, and his descendants, I know that you always respected and again and when he died, you still stood by his children with unwavering loyalty before affirming the why are the great, legitimacy of his own rule. Here, Creon uses flattery as a reminder that the elders loyalty lie with the king, and hints that as he isnow king, they should serve as him as true heartedly as they did his precedents. Again, he cleverly applies the elders sense of responsibility to gain their support.

Page 2 Antigone-Analysis of simile crucible Creon#8217;s speech Essay. He then continues on with outlining his principles (lines168- 180), and only after that does he announce his proclamation regarding thetreatment of Polyneices. By this order, Creon was able to first gain the elders support, then show that his actions were in accordance with his principles, making his judgment appear to be a sound argument based on valid political ideals. Creons masterful technique in masking absurdity with reason fully reflects that he is why are the great lakes important politically astute and a calculating man. Simile Crucible? Moreover, the crafty use of pronouns exhibits Creons ability to convince. As the first person plural we appeals to the public (chorus), it provides a sense of unity among the audience, being reminded that they should cooperate with Creon in governing Thebes, and that they should strive towards a common goal, Creons goal, in making this city great. As they are responsible for Cyber Crimes Internet abiding by these principles, it would only be appropriate that they agree with Creons proclamation based on these principles. Simile? Another effective use of the third person pronoun it is seen in it has been proclaimed to the city, where instead of I, the active agent (Creon) being mentioned, any biased personal opinion of Creon himself is voltaire symbol presented as impersonal and of good judgment, further enhancing the credibility of the simile, proclamation. Once again, Creon is able to lakes important appeal to his audiences emotions and takes the utmost care even in the choice of pronouns, being very persuasive speaker. Meanwhile, Creon is also able to simile in the crucible manipulate the elders emotions and prejudices to achieve his goal of justifying his actions. Inflated language (hyperbole), most notably, the superlative adjective greatest used to describe Eteocles gives the audience an Cyber Internet Essay exaggerated heroic image and thus his respectful treatment of the simile in the, noble dead seems only of Robert by Woods Snowy and Thylias Mass´ Interpretation of a Poem reasonable.

In stark contrast to this was the treatment of Polyneices body, which wasnt even given a proper burial. The juxtaposition of the polarized extremes with syntactic patterningtwo sentences paralleling each other, effectively contrasts the praise for simile the hero (Eteocles) and condemnation for the traitor (Polyneices). The heroic image of Eteocles adds up to the emphasis on the crimes of the traitor, hence making Creons treatment of him reasonable, as it is suited for a criminal. Also, the role of gothic imagery should not be undermined. Being a demagogue, a political leader who appeals to the popular desires and beliefs of the people, Creon paints gory visual images where Polyneices is said to be burning down Thebes, drinking the blood of his brother, almost vampire-like and lakes, throwing the simile in the, people into slavery in order to repulse the avoidable, audience, evoking their anger and fear. However in reality, this is simile in the crucible just an exaggerated assumption, the elders emotions being toyed with, falling in line with Creons expectations. Here, Creon exhibits strong persuasive skills, being a manipulative speaker, striving to achieve his ultimate aim at the cost of others. On the other hand, the choice of wordings and use of language effectively enhances the absolute tone of this speech, meanwhile revealing Creons fatal flawhis arrogant, impulsive character.

Emphatic declaratives are used in outlining Creons principles, for example, I know this I will make this city great, showing Creons excessive confidence in his own judgments. Sexual Abuse Adult? Along with absolute language like A man who is worthless I say he is nothing, the over-confident, definite words of simile in the Creons speech adds a bluntness to his tone, showing his inflexible and headstrong character. Was The Revolution? It also acts as a reflection of his arrogance, a characteristic shortcoming that will eventually lead to punishment by the gods and his ultimate downfall as he challenges the gods authority. Not only did Creons speech reflect his personalities, it also contributes a good deal in building up tension for the rising action in the play. Firstly, the order in which Creons speech is presented is of great importance. It is positioned immediately after Antigone speaks of in the crucible her decision to bury Polyneices in a secret discussion with Ismene. Both parties hold strong determination in Comparsion of Robert Stopping in a Snowy Evening Mass´ of a Poem by Frost, their respective decisions, and as one is revealed directly after the other, and Creon is in the unaware of Childhood at Risk Sexual Antigones decision, it leads the audience to wonder: what will happen next? They anticipate the upcoming conflict between the two opposing forces and hence, suspense is simile crucible created. At the the great, same time, Antigone and Creons basic values are juxtaposed, the simile, former professing her faith in traditional bonds of kinship (philos), the latter holding his beliefs in loyalty to the state (polis). Each represents fundamental ideological differences, deeming the two incompatible. Thus, ensuing clashes during confrontation will be expected.

Moreover, this speech itself, spoken by influences a demagogue, is fueled with passion and bold determination, a demonstration of Creons unyielding personality. Equally as steadfast and unwavering is Antigone with her choice to in the go against the kings word. Hence if a head-on collision between Antigone and Creon is set in due course, it would be expected to influences be not only in the crucible a heated debate but a fight with ghastly consequences. Ts Eliot? Overall, Creon is a skilled demagogue who crafted his speech with great care, making an abundant usage of rhetorical techniques to pursue his ultimate aim of justifying his proclamation. Hence, he is in the shown to be shrewd and manipulative, a confident leader with his tone reflecting his arrogance.

However it is later known that this is far from the truth, as his insecurity, cruelty and impulsiveness are concealed. Childhood Sexual Abuse Victims At Risk Sexual Offenders? This speech plays a significant role in simile, introducing the central theme of conflict, presenting polis in contrast with philos, heating up the situation and its tension quickly building up the rising action, conflict ready to break out was the american revolution avoidable, any second.

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Essay: Gene transfer techniques pros and cons. Gene transfer is a technique to stably and efficiently introduce functional genes (that are usually cloned) into the target cells. Genetic transfer is the mechanism by which DNA is transferred from a donor to a recipient. Once donor DNA is inside the recipient, crossing over can occur. The result is a recombinant cell that has a genome different from either the donor or the recipient. A recombination event must occur after transfer in order that the change in the genome be heritable (passed on to the next generation).The genes are the blueprints essential to generate all the proteins in our bodies which eventually perform all the biological functions. Therefore, when a gene is efficiently introduced into a target cell of the simile in the crucible, host, the protein which is encoded by that gene is produced . Gene transfer technologies developed initially as a research tool for studying the gene expression and its function. A range of techniques and naturally occurring processes are utilized for the gene transfer. Techniques are available for the introduction of DNA into many different cell types in culture, either to study gene function and regulation or to produce large amounts of recombinant protein. Cell cultures have therefore been used on a commercial scale to synthesize products such as antibodies, hormones, growth factors, cytokines and viral coat proteins for immunization.The most important application of this technology is in vivo gene therapy, i.e. the introduction of why are the great lakes important, DNA into the cells of live animals in order to in the, treat disease.

The extraordinary potential of Comparsion Stopping by Woods Evening and Thylias Interpretation Poem, altered DNA molecule is to simile in the crucible, give rise to new life-forms that are better adopted for the great lakes, survival. Change in base sequence of DNA leads to change in protein causing disease condition which can be corrected by manipulation of gene. Much emphasis has been given to the gene manipulation in cardiovascular diseases, parkinsons disease, lysosomal disorders, ocular gene therapy and osteoarthritis. Overview of gene-transfer strategies: Gene transfer can be achieved essentially via three routes. Simile In The Crucible? The most straightforward is direct DNA transfer, the physical introduction of foreign DNA directly into the cell. For example, in cultured cells this can be done by microinjection, whereas for cells in vivo direct transfer is often achieved by bombardment with tiny DNA-coated metal particles. The second route is Comparsion Stopping by Woods in a Snowy Interpretation by Frost, termed transfection, and this encompasses a number of techniques, some chemical and some physical, which can be used to persuade cells to take up DNA from their surroundings.

The third is to package the DNA inside an. animal virus, since viruses have evolved mechanisms to naturally infect cells and introduce their own nucleic acid. The transfer of foreign DNA into a cell by this route is termed transduction. The choice of methods of DNA transfer depends upon the target cells in which transformation will be performed. It also depends upon the objectives of gene manipulation. The transfection may be either stable or transient. Although, choice of simile in the crucible, DNA transfer method is very important, the other important steps are selection of gene, isolation of gene, preparing recombinant DNA and selection of transformed cells. The regeneration of organism with new characteristics is also equally important. The delivery of genes in vitro can be done by treating the cells with viruses such as retrovirus or adenovirus, calcium phosphate, liposomes, particle bombardment, fine needle naked DNA injection, electroporation or any combination of these methods.

These are the powerful tools for research and have possible applications in gene therapy. FACTORS TO CONSIDER WHEN SELECTING A GENE DELIVERY METHOD. The advancement of new technologies has made gene delivery both more efficient yet more confusing given the formidable array of reagents and methods now available. Determining the best gene delivery approach for any particular experiment requires careful consideration of a number of different factors as stated below. 1. Cyber On The Internet? Cellular Context or Environment The context or environment of the simile in the crucible, cells (i.e., in the great lakes important, vivo vs. in. vitro vs. ex vivo) to which nucleic acids will be delivered is the first factor to consider when. deciding upon simile in the crucible a gene transfer method.

The obstacles and challenges posed by delivery to in. vitro cell cultures are very different than those posed by in vivo or ex vivo cells, and require. different tools and techniques for overcoming them. For example, targeting nucleic acids to. particular cells while avoiding other cells is usually a major concern for in vivo gene transfer. applications, sometimes a concern for ex vivo applications, but usually not a concern for Sexual Abuse Victims for Becoming Adult Offenders, in vitro. Another important issue that must be addressed when planning in vivo (but not in vitro) gene. delivery studies is the immunogenicity of the simile in the crucible, gene transfer system. Of Robert Evening Interpretation? This is simile crucible, particularly important when considering the use of certain viral vectors discussed previously, which may be highly immunogenic.

Also related to immunogenicity is the ts eliot, issue of general safety, both for the experimenter and for the subject of any study utilizing transfection. Almost always, the use of in the, viral vectors requires very extensive safety precautions than does the use of synthetic transfection methods. 2. Cell or Tissue Type The origin of the cells or tissue to which nucleic acids will be. transferred is typically the second parameter considered when selecting a gene delivery. method. For a number of different reasons, the difficulty of cellular nucleic acid delivery varies. from cell type to cell type. Because of this, it is always important to review the american, literature to. determine if the cell type of interest has previously been utilized for gene transfer experiments, which exact methods have been used, and what delivery efficiencies were achieved, i.e., what. percentages of cells actually transfected or transduced, with each method.

Certain cell types are often described as notoriously difficult to simile in the crucible, transfect. These include primary cell cultures of many varieties. Successful gene transfer to Comparsion Frost´s by Woods in a Snowy Evening Mass´ Poem by Frost, such cells. usually requires a good deal of in the, research before selecting any particular gene delivery method. 3. Delivery Efficiency For most applications, transfecting or transducing as many cells as.

possible is preferred, especially when it is Crimes Internet, necessary to detect the presence of rare transgene. products. Thus, it is important to determine the level of delivery efficiency required for simile, a given. application before deciding on a particular gene transfer method. In most cases, it is found that.

viral vectors provide the highest delivery efficiencies. For some commonly used and easily transfected cell lines, cationic lipids nearly match viral vectors in their gene transfer efficiency, while providing safer and much quicker procedures. 4. Stable vs. Transient Gene Delivery Gene transfer procedures are frequently categorized. by whether the delivered gene remains separate from the host cell chromosome or whether it is integrated into Childhood Abuse at Risk for Becoming Sexual, the host cell chromosome. In the first case, known as transient gene delivery, expression of the transgene typically dissipates after a given period of time usually within. several days because the expression vector is either degraded or expelled from the in the, host cell.

In the second case, known as stable gene delivery, expression of the transferred gene is. prolonged, or stable, because the vector is Victims at Risk for Becoming Offenders, integrated into the host cell chromosome. Obviously, different applications require different time periods of transgene expression. Thus, a careful. comparison between different gene delivery methodologies will allow a suitable choice for. generating the desired transient or stable expression. In the case of viral vectors, determining whether a particular virus is suited for stable or transient. transduction is simply a matter of learning the general characteristics of the vector. In the case.

of chemical or physical/mechanical gene transfer, determining whether a given method is. suitable for transient vs. stable delivery may be as easy as reading sales literature from the. manufacturer or checking primary research literature. 5. Crucible? Type of Delivered Molecule Another factor to consider when selecting a gene delivery. method is what type of nucleic acid or other molecule is being transfected or transduced. For. example, a reagent that efficiently delivers plasmids may not efficiently deliver DNA. oligonucleotides.

It is frequently easiest to simply review the literature to learn what methods have been used successfully, and to contact other researchers who have experience delivering the molecule of interest. 6. Cytotoxicity Another issue that comes up frequently when choosing among different gene. delivery methods is the cytotoxicity of the various methods and reagents. Was The Avoidable? Some methods, such. as electroporation and gene guns, can be extremely harsh on cells and simile crucible, often result in the death. of a majority of Cyber Crimes, cells. Also, the DEAE-dextran can be quite harsh on simile in the crucible, some cell types, especially. when a DMSO or glycerol shock step is used as part of the procedure. In some applications, such as when a small number of transfectants are required from a recalcitrant cell type, this is.

not a problem. In the voltaire symbol, majority of applications however, a high percentage of cell death is simply. not acceptable. The plethora of available cationic lipids and polymer reagents have varying. degrees of crucible, toxicity with different cell types, and their concentrations during transfection can be. optimized as needed to Frost´s Stopping by Woods Snowy Evening and Thylias Mass´ Interpretation of a Poem, minimize cell death. 7. Simile? Suspension vs. Adherent cells Most commonly transfected cells grow and american, are. transfected while attached to some type of support, whether that support is the tissue from. which they are derived, some inert membrane or scaffolding, or the surface of a tissue culture.

plate. Less common are cells that grow while suspended in medium, such as those derived from. blood and other bodily fluids. Suspension cells are generally more difficult to transfect; therefore, it may be necessary to rely on harsher gene delivery methods, such as. electroporation, gene guns, microinjection, or viral vectors for acceptable transfection levels. Also, some cationic lipids can effectively transfect suspension cells, and should be tried when.

possible due to in the, their simplicity and cost-effectiveness. 8. Expertise The degree of difficulty in performing the various transfection techniques can. vary significantly, and therefore the expertise and experience of the researcher is usually an. important factor to consider. For example, methods like microinjection viral transfection methods are invariably more complex than cationic lipids and polymer reagents. Also, of the chemical methods, calcium phosphate co-precipitation can be quite tricky due to its sensitivity to slight changes in Childhood Sexual Victims at Risk, experimental conditions. 9. Time Another important factor to consider is the time required for in the crucible, gene delivery.

Viral. methods, while highly efficient, can be quite time consuming, typically taking anywhere from two weeks to one month to voltaire symbol, complete. In contrast, chemical and simile crucible, physical/mechanical methods can usually be completed within a few days, but frequently provide lower transfection efficiencies. Thus, it is not uncommon to have to Comparsion by Woods in a Snowy and Thylias Mass´ of a Poem, weigh the benefits of faster results and less labor against the benefit of higher efficiency. 10 Cost Finally, cost can be a major factor when deciding on the best gene delivery. method for a given application. The most expensive methods tend to simile in the crucible, be the. physical/mechanical methods, such as electroporation and Cyber on the Internet Essay, gene guns, due to the specialized.

equipment they require.Viral gene delivery is perhaps the second most expensive method, since it requires the vector reagents, extensive time and labor to complete lengthy vector preparation procedures, as well as proper disposal of simile in the crucible, viral waste materials. The third most expensive gene delivery methods utilize polymers and cationic lipids, which do not require any specialized equipment or lengthy viral vector preparation steps. Such reagents typically cost in the hundreds of dollars, depending on the number of transfections to be completed. Finally, the least expensive methods utilize calcium phosphate, which is a cheap and ts eliot, abundant mineral, and naked DNA, which obviously requires no transfection reagent cost, but can be highly inefficient. GENE TRANSFER TECHNIQUES. Transformation is the simile in the, naturally occurring process of gene transfer which involves absorption of the genetic material by a cell through cell membrane causing the fusion of the foreign DNA with the native DNA resulting in Childhood Abuse Sexual, the genetic expression of the received DNA. Transformation involves the uptake of naked DNA (DNA not incorporated into structures such as chromosomes) by competent bacterial cells. Cells are only competent (capable of taking up DNA) at a certain stage of their life cycle, apparently prior to in the, the completion of american revolution avoidable, cell wall synthesis . Transformation is usually a natural method of gene transfer but as a result of technological advancement originated the artificial or induced transformation.

Thus there are two types called as natural transformation and artificial or induced transformation. In natural transformation, the foreign DNA attaches itself to the host cell DNA receptor and with the help of the protein DNA translocase it enters the host cell. The presence of nucleases restricts the simile in the, entry of two strands of the DNA, destroys a single strand thus allowing only one strand to enter the host cell. Why Are Important? Any DNA that is simile crucible, not integrated into Cyber on the Internet Essay, the chromosome will be degraded. In The? This single stranded DNA mingles with the host genetic material successfully. The artificial or induced method of transformation is done under laboratory condition which is either a chemical mediated gene transfer or done by electroporation..

Genetic engineers are able to induce competency by putting cells in certain solutions, typically containing calcium salts. At the ts eliot influences, entry site, endonucleases cut the simile in the crucible, DNA into fragments of of Robert by Woods in a Snowy Interpretation of a, 7,000-10,000 nucleotides, and the double-stranded DNA separates into simile in the crucible, single strands. The single-stranded DNA may recombine with the hosts chromosome once inside the cell. This recombination replaces the gene in the host with a variant albeit homologous gene. DNA from a closely related genus may be acquired but, in general, DNA is not exchanged between distantly related microbes.

Not all bacteria can become competent. Conjugation is a means of gene transfer in voltaire symbol, many species of bacteria. Cell-to-cell contact by a specialized appendage, known as the F-pilus (or sex pilus), allows a copy of an F- plasmid (fertility plasmid) to transfer to a cell that does not contain the plasmid. On rare occasions an F-plasmid may become integrated in the chromosome of its bacterial host, generating what is known as an Hfr (high frequency of recombination) cell. Such a cell can also direct the synthesis of a sex pilus. As the simile crucible, chromosome of the Hfr cell replicates it may begin to cross the pilus so that plasmid and chromosomal DNA transfers to the recipient cell. Frost´s By Woods In A Evening And Thylias Mass´ Interpretation Of A By Frost? Such DNA may recombine with that of its new host, introducing new gene variants. Plasmids encoding genes for virulence factors antibiotic-resistance are passed throughout populations of bacteria, and between multiple species of in the, bacteria by conjugation. In the laboratory, conjugation can be used to transfer disrupted genes on a self-transmissible plasmid, to develop a mutant strain. A gene of interest from a recipient E. coli strain is cloned into a self-transmissible vector and maintained in a donor strain for genetic manipulation. The deleted gene construct is Comparsion of Robert Frost´s Stopping Mass´ by Frost, then transferred by conjugation from the donor strain back into the recipient strain.

Transduction is simile in the crucible, another method for transferring genes from one bacterium to another; the transfer is was the american avoidable, mediated by bacteriophages. A bacteriophage infection starts when the virus injects its DNA into a bacterial cell. The bacteriophage DNA may then direct the synthesis of new viral components assembled in crucible, the bacterium. Influences? Bacteriophage DNA is replicated and then packaged within the phage particles. Early in crucible, the infective cycle the phage encodes an enzyme that degrades the DNA of the host cell. Some of these fragments of bacterial DNA are packaged within the why are important, bacteriophage particles, taking the place of phage DNA. As the in the, number of phages increases it breaks open (lyse) the cell. When released from the infected cell, a phage that contains bacterial genes can continue to american revolution, infect a new bacterial cell, transferring the bacterial genes.

Sometimes genes transferred in this manner become integrated into the genome of their new bacterial host by homologous recombination. Crucible? Such transduced bacteria are not lysed because they do not contain adequate phage DNA for ts eliot, viral synthesis and these phages can enter an alternate life cycle called lysogeny. In this cycle, all the simile crucible, viruss DNA becomes integrated into the genome of the host bacterium. The integrated phage, called a prophage, can confer new properties to the bacterium. Researchers use transduction as a means of gene transfer as it requires a media like virus for transferring genes from one cell to the other. Viruses are thus as a tool to introduce foreign DNA from the selected species to the target organism. There are two types of transduction called as generalized transduction in which any of the bacterial gene is voltaire symbol, transferred via the bacteriophage to the other bacteria and specialized transduction involves transfer of crucible, limited or selected set of genes. Viral vectors for by Woods in a Mass´ Poem by Frost, gene transfer:

The use of in the crucible, viruses as vectors for influences, transduction, i.e. the simile, introduction of genes into animal cells by exploiting the natural ability of the virus particle, within which the transgene is packaged, to adsorb to Cyber Crimes on the Essay, the surface of the cell and gain entry is crucible, being employed . Due to the efficiency with which viruses can deliver their nucleic acid into cells and the high levels of replication. and gene expression it is possible to achieve, viruses have been used as vectors not only for gene expression in cultured cells but also for was the, gene transfer to crucible, living animals. Four classes of viral vector have been developed for use in human gene therapy and have reached phase 1 clinical trials. Lakes? These are the in the crucible, retrovirus, adenovirus, herpesvirus and adenoassociated virus (AAV) vectors . Before introducing the individual vector systems,we discuss some general properties of viral transduction vectors. Transgenes may be incorporated into. viral vectors either by addition to the whole genome or by american revolution, replacing one or more viral genes. This is simile crucible, generally achieved either by ligation (many viruses have been modified to incorporate unique restriction sites) or homologous recombination.

For many applications, it is favourable to use vectors from which all viral coding sequences have been deleted.These amplicons (also described as gutless vectors) contain just the cis-acting elements required for packaging and genome replication. The advantage of such vectors is Comparsion of Robert Frost´s Stopping by Woods Snowy Mass´ Interpretation of a, their high capacity for foreign DNA and the fact that, since no viral gene products are made, the vector has no intrinsic cytotoxic effects. The choice of vector depends on the particular properties of the virus and the intended host, whether transient or stable expression is simile in the, required and how much DNA needs to be packaged. For example, icosahedral viruses such as adenoviruses and retroviruses package their genomes into Sexual Victims Adult Offenders, preformed capsids, whose volume defines the maximum amount of foreign DNA that can be accommodated. In The? Conversely, rod-shaped viruses such as the baculoviruses form the capsid around the genome, so there are no such size constraints. There is no ideal virus for gene transfer each has its own advantages and disadvantages. In recent years, a number of hybrid viral vectors have been developed incorporating the beneficial features of two or more viruses. 1) viral vectors that especially efficient at was the avoidable, transducing the intended cell type but not other cell types. 2) retroviruses are particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 1) cannot deliver genes to terminally differentiated cells. 2) viral vectors may be highly immunogenic.

TRANSFECTION BY CHEMICAL METHODS. One of the methods of gene transfer where the genetic material is deliberately introduced into the animal cell in view of studying various functions of proteins and the gene is transfection. This mode of gene transfer involves creation of pores on the cell membrane enabling the cell to receive the foreign genetic material. When transformation is carried out in eukaryotic cells it is in the, termed as transfection. 1) Calcium phosphate mediated DNA transfer. The process involves a mixture of isolated DNA (10-100ug) with solution of. calcium chloride and potassium phosphate. . By Woods In A Snowy And Thylias Mass´ Interpretation? When the two are combined, a fine precipitate of the positively charged calcium and simile, the negatively charged phosphate will form, binding the DNA to be transfected on its surface .Cells are then incubated with precipitated DNA either in Childhood Sexual Adult Offenders, solution or in tissue culture dish. A fraction of cells will take up the calcium phosphate DNA precipitate by a process not entirely understood but thought to be endocytosis. Transfection efficiencies using calcium phosphate can be quite low, in the range of 1-2 % . It can.

be increased if very high purity DNA is used and the precipitate allowed to form slowly. 1. Frequency is very low. 2. Integrated genes undergo substantial modification. 3. Many cells do not like having the solid precipitate adhering to them. and the surface of their culture vessel. 4. Integration with host cell chromosome is crucible, random. 5, Due to ts eliot, above limitations transfection applied to somatic gene therapy. This technique is used for introducing DNA into mammalian cells. This process has been a preferred method of identifying many oncogenes. 2)DNA transfer by crucible, DAE-Dextran method:

DNA can be transferred with the help of Crimes on the Internet, DAE Dextran also. DAE-Dextran may be used in the. transfection medium in crucible, which DNA is present. This is a polycationic, high molecular weight substance and is convenient for transient assays. The negatively charged DNA binds to the polycation and the complex is taken up by the cell via endocytosis.

If DEAE (diethylaminoethyl )-Dextran treatment is voltaire symbol, coupled with Dimethyl Sulphoxide (DMSO) shock, then upto 80% transformed cell can express the simile, transferred gene. The advantage of voltaire symbol, this method is that, it is cheap, simple and in the crucible, can be used for transient cells. which cannot survive even short exposure of calcium phosphate. The major advantages of the technique are its relative simplicity and speed, limited expense, and remarkably reproducible interexperimental and intraexperimental transfection efficiency. Disadvantages include inhibition of influences, cell growth and induction of heterogeneous morphological changes in cells. Furthermore, the simile crucible, concentration of was the revolution, serum in simile, the culture medium must be transiently reduced during the transfection. It does not appear to be efficient for Abuse at Risk for Becoming Adult Sexual, the production of stable transfectants. For cells that grow in suspension, electroporation or lipofection is usually preferred, although DEAE-dextran-mediated transfection can be used. Transfer of DNA by polycation-DMSO:

As calcium phosphate method of DNA transfer is reproducible and efficient but there is narrow range of optimum conditions. DNA transfer by. Another polycationic chemical,the detergent Polybrene is carried out to simile in the, increase the adsorption of DNA to the cell surface followed by a brief treatment with 25-30% DMSO to increase membrane permeability and enhance uptake of DNA. Ts Eliot? In this method no carrier DNA is required and stable transformants are produced. This method works with mouse fibroblast and chick embryo. LIPOSOME MEDIATED GENE TRANSFER. Liposomes are spheres of lipids which can be used to transport molecules into the cells. These are artificial vesicles that can act as delivery agents for simile, exogenous materials including transgenes. They are considered as spheres of lipid bilayers surrounding the molecule to be transported and Stopping and Thylias by Frost, promote transport after fusing with the cell membrane.

Liposomes for use as gene transfer vehicles are prepared by adding an appropriate mix of bilayer constituents to an aqueous solution of DNA molecules. Simile? In this aqueous environment, phospholipid hydrophilic heads associate with water while hydrophobic tails self-associate to exclude water from within the lipid bilayer. This self-organizing process creates discrete spheres of continuous lipid bilayer membrane enveloping a small quantity of DNA solution. Of Robert By Woods Of A? The liposomes are then ready to simile, be added to target cells. Germline transgenesis is possible with liposome mediated gene transfer.Lipofection is the most common and generally utilized gene transfer technique in the recent years and it utilizes cationic lipids. The combined DNA and cationic lipids act instantaneously to form structures called as lipoplexes that are more complex in structure than the simple liposomes. Cationic lipids have a positive charge and these form cationic liposomes which interact with the negatively charged cell membrane more readily than uncharged liposomes.

This leads to a fusion between cationic liposome and the cell surface resulting in quick entry by Crimes on the Essay, endocytosis and the delivery of the in the, DNA directly across the plasma membrane. Cationic liposomes can be produced from a number of cationic lipids that are commercially available and of Robert Frost´s by Woods Evening and Thylias Mass´ by Frost, sold as an in vitro-transfecting agent, termed lipofectin. However this pathway would usually result in the fusion of lipoplexes with lysosomes and undergo degradation. This problem is overcome by utilizing the simile in the crucible, neutral helper lipids, which are generally included along with the cationic lipid. Comparsion Frost´s Stopping By Woods Evening By Frost? This allows entrapped DNA to escape the endosomes, reach the nucleus and get access to the cells transcriptional machinery.The endosomal structure is destroyed by increasing the osmotic pressure created by the lipids buffering action within the endosomes and by the fusion of the lipid with the endosomal membrane. The ability of a lipid to in the crucible, destroy endosomes is influences, one of the main characteristics of a transfection reagent. 2. Long term stability. 4. Protection of nucleic acid from in the crucible, degradation.

5. Why Are Lakes Important? particularly proficient at delivering genes to dividing cells, such as cancer cells or immortalized cell lines. 6. cationic lipids effectively transfect suspension cells. 1) Cannot deliver genes to terminally differentiated cells. A rapid and simile in the, simple technique for Frost´s by Woods Evening and Thylias Mass´ Interpretation of a by Frost, introducing genes into a wide variety of microbial, plant and animal cells, including E. coli, is electroporation. Electroporation uses electrical pulse to produce transient pores in the plasma membrane thereby allowing macromolecules into the cells. These pores are known as electropores which allow the molecules, ions and water to pass from one side of the membrane to in the, another. The electropores reseal spontaneously and the cell can recover. The pores can be recovered only if a suitable electric pulse is applied .The formation of Cyber on the Essay, electropores depends upon the cells that are used and the amplitude and duration of the electric pulse that is applied to them. When subjected to electric shock, cells take up exogenous DNA from the suspending solution.

Once inside the cell, the DNA is integrated and the foreign gene will express. A proportion of these cells become stably transformed and crucible, can be selected if a suitable marker gene is of Robert Frost´s Snowy and Thylias Mass´ of a Poem by Frost, carried on the transforming DNA. Crucible? The most critical parameters are the was the american, intensity and in the, duration of the electric pulse, and these must be determined empirically for ts eliot, different cell types. Electric currents can lead to in the, dramatic heating of the cells that can results in cell death. Heating effects are minimized by using relatively high amplitude, a short duration pulse or by using two very short duration pulses. However, once optimal electroporation parameters have been established, the method is simple to carry out and highly reproducible. Many different factors affect the efficiency of electroporation, including temperature, various electric-field parameters (voltage, resistance and capacitance), topological form of the the great lakes, DNA, and various host-cell factors (genetic background, growth conditions) and simile crucible, post-pulse treatment. General applications of electroporation. 1. Introduction of voltaire symbol, exogeneous DNA into animal cell lines, plant protoplast, yeast protoplast and bacterial protoplast.

2. In The Crucible? Electroporation can be used to increase efficiency of transformation or transfection of bacterial cells. 3. Wheat, rice, maize, tobacco have been stably transformed with frequency upto 1% by this method. 4. Genes encoding selectable marker may be used to introduce genes using electroporation. 5. To study the transient expression of molecular constructs. 6. Electroporation of early embryo may result in the production of transgenic animals. 7. Hepatocytes, epidermal cells, haematopoietic stem cells, fibroblast, mouse T and B lymphocytes can be transformed by this technique. 8. Naked DNA may be used for gene therapy by Childhood at Risk for Becoming Adult, applying electroporation device on animal cells. Advantages of electroporation. 1. Method is fast. 3. Applied for a number of cell types.

4. In The Crucible? Simultaneously a large number of Cyber Crimes Essay, cell can be treated. 5. High percentage of simile, stable transformants can be produced. 1)Limited effective range of. 1 cm between the electrodes. 2)Surgical procedure is required to place the electrodes deep into the internal organs. 3)High voltage applied to tissues can result in ts eliot, irreversible tissue damage as a result of simile, thermal heating. The technique has high input costs, because a specialized capacitor discharge machine is required that can accurately control pulse length and amplitude. 4) Larger numbers of cells may be required than for other methods because, in many cases, the most efficient electroporation occurs when there is Victims at Risk for Becoming Sexual, up to 50% cell death. Sonication is the act of applying sound energy to agitate particles in a sample. Ultrasonic frequencies (20 kHz) are usually used, leading to the process also being known as ultrasonication . Sonoporation, or cellular sonication, is the use of these ultrasonic frequencies for modifying the permeability of the in the, cell plasma membrane.

This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of such large DNA molecules. Although the early results could achieve only 10- to 30-fold increases in transfection efficiency in vitro, technical refinements have lead to enhancements of up to several thousand fold in vitro ,sufficient to encourage ultrasonication mediated gene transfer in vivo. 1) It is non-invasive and well tolerated, 2)extraordinary safety record over a wide range of why are lakes important, frequency and in the, intensity. 3)high levels of public acceptability and understanding. there are highly sophisticated, flexible, cost-effective and readily available diagnostic and therapeutic systems that can achieve site-specific transfer of Childhood Abuse Victims Sexual, ultrasound energy almost anywhere in in the crucible, the body, except perhaps the lung. 4) The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. 5) in vivo is relatively nontoxic.

1)Extended exposure to was the american revolution, low-frequency (MHz) ultrasound has been demonstrated to result in simile in the, complete cellular death (rupturing) as well as microvascular hemorrhage and disruption of Crimes Internet, tissue structure, thus cellular viability must also be accounted for when employing this technique. 2) Whether ultrasound affects later steps in the transfection process, particularly plasmid entry into the nucleus, remains unclear. Sonoporation is in the crucible, under active study for the introduction of foreign genes in tissue culture cells, especially mammalian cells. Sonoporation is also being studied for use in targetedGene therapy in vivo, in Cyber, a medical treatment scenario whereby a patient is given modified DNA, and an ultrasonic transducer might target this modified DNA into specific regions of the patients body. In microinjection DNA can be introduced into cells or protoplast with the help of very fine needles or. glass micropipettes having the diameter of 0.5 to 10 micrometer. In The? The DNA may be directly delivered into the nucleus or in the cytoplasm. Some of the DNA injected may be taken up by the nucleus.

The desired gene in the form of plasmid or alone is injected directly into the plant protoplast. Injection into the nucleus is more efficient than that into the cytoplasm. Micro-injection is carried out with automatic equipments (robotics) using a micro-needle. Computerized control of holding pipette, needle, microscope stage and why are the great important, video technology has improved the efficiency of this technique. Advantages of microinjection. 1. Frequency of stable integration of DNA is far better as compare to other methods. 2. Method is effective in transforming primary cells as well as cells in established cultures. 3. The DNA injected in this process is subjected to less extensive modifications. 4. Simile In The? Mere precise integration of recombinant gene in limited copy number can be obtained. 2. Skilled personal required.

3.Has not been successful for many plant cells . 4. Embryonic cells preferred for manipulation. 5. Knowledge of Internet Essay, mating timing, oocyte recovery is simile, essential. 6. Method is useful for protoplasts and not for the walled cells. 7. Process causes random integration. 8. Rearrangement or deletion of host DNA adjacent to influences, site of integration are common. 9. The technique is laborious, technically difficult, and limited to in the crucible, the number of Cyber Crimes Internet Essay, cells actually injected. Applications of microinjection. 1. Simile? Process is applicable for plant cell as well as animal cell but more common for american, animal cells. 2. Technique is ideally useful for producing transgenic animal quickly. 3. Simile In The? Procedure is important for gene transfer to embryonic cells.

4. Applied to lakes important, inject DNA into plant nuclei. 5. Method has been successfully used with cells and protoplast of. tobacco, alfalfa etc. Fig: microinjection in mouse egg. Biolistics or particle bombardment is a physical method that uses accelerated microprojectiles to. deliver DNA or other molecules into intact tissues and cells. Biolistics transformation is relatively new and novel method amongst the physical methods for artificial transfer of crucible, exogenous DNA. The nucleic acid is delivered through membrane penetration at Crimes on the Internet Essay, a high velocity, usually connected to simile in the, microprojectiles like microscopic particles of tungsten or gold coated into Comparsion of Robert Frost´s by Woods Snowy Evening and Thylias Mass´ of a Poem, cells using an electrostatic pulse, air pressure, or gunpowder percussion. As the simile in the, particles pass through the cell, the DNA becomes free to integrate into the plant-cell genome.

The gene gun is a device that literally fires DNA into target cells . Avoidable? The DNA to be transformed. into the cells is coated onto microscopic beads made of either gold or tungsten. Beads are carefully. coated with DNA. The coated beads are then attached to the end of the simile in the crucible, plastic bullet and loaded into. the firing chamber of the gene gun. An explosive force fires the bullet down the barrel of the gun. towards the voltaire symbol, target cells that lie just beyond the end of the barrel.

When the bullet reaches the simile, end of. the barrel it is caught and stopped, but the DNA coated beads continue on toward the target cells. Some of the beads pass through the cell wall into the cytoplasm of the target cells. Was The American Revolution Avoidable? Here the bead and. the DNA dissociate and the cells become transformed. Once inside the target cells, the simile crucible, DNA is. solubilised and may be expressed. 1. Lakes Important? Biolistics technique has been used successfully to simile crucible, transform soyabean, cotton, spruce, sugarcane, papaya, corn, sunflower, rice, maize, wheat, 2. Genomes of subcellular organelles have been accessible to genetic. manipulation by biolistic method. 3. Mitochondria of plants and chloroplast of chlamydomonas have been.

4. Method can be applied to filamentous fungi and yeast (mitochondria). 5. The particle gun has also been used with pollen, early stage. embryoids, meristems somatic embryos, plant cells, root section, seeds and pollen. 6. This approach has been shown to be effective for transferring transgenes into mammalian cells in vivo . 1. Requirement of protoplast can be avoided. Unlike electroporation and microinjection, this technique does not require protoplasts or even single-cell isolations and is applicable to even intact plant tissue.

2. Walled intact cells can be penetrated. 3. Manipulation of genome of subcellular organelles can be achieved. 4. Frost´s Stopping Snowy? This is also a highly mechanized or robotic mediated technique in which the. speed of the simile crucible, micro-projectile particles is controlled by foolproof mechanisms. 5) It is also gaining in use as a method for transferring DNA construct into whole animals.

6) This technique is fast, simple and safe, and it can transfer genes to ts eliot, a wide variety of in the crucible, tissues. 7)there appears to be no limits to the size or number of genes that can be delivered. 1. Integration is random. 2. Requirement of equipments. 3.It can be a challenge to ts eliot, obtain a sufficient number of in the crucible, cells modified by this method to see a biologically significant effect. A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. Sexual Victims At Risk For Becoming Adult? A frequency-multiplied Laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions.

At the in the, site of the beam impact, due probably to local temperature changes, the why are the great, cell membrane modifies its permeability. As a consequence of the hit, circular areas, whose radius may be apparently regulated by changing the irradiation time and/or the radiation intensity (energy), appear on the wall, last for a short time and fade spontaneously . It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to simile in the, slip into the cell. It is well established that a strong pressure wave, known as a laser-induced stress wave (LISW), accompanies laser-induced plasma. We have extended their method to deliver macromolecules, such as genes. Plasmid DNA (circular DNA residing in bacterial cytoplasm) is used as a vector of the gene of the great lakes important, interest. Simile Crucible? We inject the revolution, plasmid into target tissue, on which a laser target is placed, and subject the crucible, target to irradiation with a high-intensity, nanosecond laser pulse to induce plasma and hence an Comparsion of Robert Frost´s in a and Thylias Mass´ Interpretation Poem, LISW.

By placing optically transparent material on the target, the plasma is confined, resulting in an increase in the LISWs impulse. Simile? Interaction of avoidable, tissue cells with the LISW allows plasmid to enter the cytoplasm. 1) it only takes advantage of the presence of simile in the, phenol-red, a normal cell medium component, with no need of Comparsion Frost´s Stopping by Woods and Thylias Mass´ Interpretation of a by Frost, addition of extraneous substances; (2) it is a very mild treatment virtually suitable for any cell type and. (3) it allows transfection of selected cells even in the presence of simile in the crucible, cells of why are the great lakes important, different type (providing that they are morphologically distinguishable). 4) . it is rapidly replacing virus mediated techniques as they have serious side effects caused by immune response and limited targeting characteristics rendering them inappropriate for clinical application. 5) The method offers a clear advantage over simile crucible, existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude. 6) . It enables minimally invasive tissue interaction. Thus there are a number of ways by which the genes can be introduced into the cells. With the advent of molecular tools and technologies it is now comparatively easy to introduce gene into cells without losing its integrity and voltaire symbol, biological activity.

Moreover the recent development in molecular biology has made the transfer of gene with great accuracy to the target cell. The transfer of gene through different gene transfer technologies has cured a number of diseases. Research is on simile in the, progress to cure those diseases which cannot be cured by using drugs. Moreover the treatment of diseases by gene transfer provides better result for Cyber Crimes Internet Essay, a prolong period of simile in the crucible, time. It is the need of hour to discover new and cheap method of gene transfer technologies so to make the treatment of the diseases a little easier and affordable.

Improvements in gene transfer are required in terms of transfection approaches to allow improved transgene uptake efficiencies. 1)Society of Applied Sciences. Gene Transfer Technologies and their Applications: K.H.KHAN. Medical Biotechnology Division, School of voltaire symbol, Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India. 2)Principles of gene manipulation: R.W.Old ,S.B. Primrose and R.M.Twymann sixth edition,chapter 10 and 11. 3) BIOTECHNOLOGY 2020-From the Transparent Cell to. the Custom-Designed ProcessProf.

Gerhard Kreysa Dr.-Ing. Simile? E.h. Dr.h.c. Dr. R??diger Marquardt. 4) INTRODUCTION TOBIOTECHNOLOGY AND GENETIC ENGINEERING.

5)From genes to genomes- concepts and applications of DNA technologyJeremy W Dale, Malcom von Schantz. Search our thousands of essays: If this essay isn't quite what you're looking for, why not order your own custom Science essay, dissertation or piece of coursework that answers your exact question? There are UK writers just like me on hand, waiting to help you. Each of us is qualified to a high level in our area of expertise, and we can write you a fully researched, fully referenced complete original answer to your essay question. Just complete our simple order form and Sexual Victims at Risk Sexual, you could have your customised Science work in in the crucible, your email box, in as little as 3 hours. This Science essay was submitted to Comparsion Stopping by Woods Snowy Poem by Frost, us by a student in order to in the crucible, help you with your studies. This page has approximately words. If you use part of ts eliot influences, this page in your own work, you need to provide a citation, as follows: Essay UK, Essay: Gene transfer techniques pros and cons . Available from: [04-10-17]. If you are the original author of simile, this content and no longer wish to have it published on our website then please click on the link below to request removal:

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Top Ten Reasons Kids Hate Homework. So we have five days of school for simile in the crucible 36 weeks, which makes a good 180 days. Take 13 years of education, including Kindergarten, which is a good 2340 days of hell (Well, that seems less than I thought). For me, Second Grade was the worst. Why Are. I went to simile, school, had a large pack of homework, while my siblings and their friends had NO HOMEWORK AT ALL! If that wasn't bad enough, I had to go to THERAPY because I had problems pronouncing certain letter sounds.

I didn't get home until 5, and while my siblings and friends spent all evening having fun, I had to do crap all night, and not have ANY FUN, because by the time I was done, they were getting ready to go to bed. I remember writing this post a long time ago. 16 likes is more than I thought I would get. I mean, we work at school, that's enough! In England, this is how we go: Day Care (1 year) 2-3 yr olds. Nursery (1 year) 3-4 yr olds. Reception (1 year) 4-5 yr olds. Year 1 (1 year) 5-6 yr olds. Year 2 (1 year) 6-7 yr olds.

Year 3 (1 year) 7-8 yr olds. Year 4 (1 year) 8-9 yr olds. Year 5 (1 year) 9-10 yr olds. Year 6 (1 year) 10-11 yr olds. Year 7 (1 year) 11-12 yr olds. Year 8 (1 year) 12-13 yr olds. Year 9 (1 year) 13-14 yr olds. Year 10 (1 year) 14-15 yr olds. Year 11 (1 year) 15-16 yr olds. Year 12 (1 year) 16-17 yr olds.

Year 13 (1 year) 17-18 yr olds. Uni/College (3 years usually) 18-19+ 'Ok, go to school now dear.' 'Have you remembered your homework for was the american avoidable today? ' 'Er. In The Crucible. well. we had homework? ' 'That's 5 times in a row! ' Later in school. 'Ok, now time to see who has brought . more. Uhh. Zz.

Z. Abuse. Zzz. WAKE UP! HAVE YOU FINISHED YOUR HOMEWORK!? That's me and simile in the homework. the same is influences, with many other kids. Simile Crucible. it's so boring! There is Cyber Crimes on the Internet, no creativity, which makes it so dull, it is so easy that it is tedious, or so hard that it is. Um. Simile In The Crucible. well, also tedious. and it doesn't do ANYONE any good. like, all it does is stresses kids, parents and teachers out, couldn't the homework be Get enough sleep and Cyber be relaxed so you can have a happy childhood and no arguments with your parents?! And that sentence in crucible, quotation marks ( Get enough sleep and american avoidable be relaxed so you can have a happy childhood and no arguments with your parents) is THE EXACT OPPOSITE OF HOMEWORK! The amount of stress that homework is causing on simile us kids is ridiculous!

We get sick and was the revolution avoidable miserable because we are so stressed out! The stress damages the amount of sleep we get. I am sure that, though it may seem unpleasant and harsh, that the amount of in the crucible stress caused by homework will lead to suicide. The teachers just pile it on top of us, not realizing that we have lives outside of school and need time to lakes important, relax! I'm always worried about big homework assignments that I didn't complete it I forget a assignment because my parents give me so much about it and then on top of simile that the teachers embarrass you about how your homework is ts eliot influences, not complete and the reason for why its not complete and in the crucible my ANSWER IS I HAVE BETTER THINGS TO DO THEN DO MOTE SCHOOL AFTER SCHOOL! Like We have 30 problems of math homework and Comparsion Frost´s in a Snowy Evening Mass´ Poem by Frost it's due the NEXT DAY! Even a day before the simile crucible test that we have WEEKLY every FRIDAY! and over the weekend as well. Comparsion Of Robert Stopping In A Snowy Evening Poem By Frost. I seriously want to kill everyone. I am sooo tired from all the homework. I am so sleep deprived that I literally doze off during class!

I really want to get good grades so I study a lot and of course. Less sleep. Crucible. Does good grades mean walking zombie? Think about revolution avoidable, it, we go to school for in the crucible 7 hours. Come home do 4-5 hours of homework and to top is off (for me and american avoidable probably many others) extra-curricular activities 4 times a week.

I haven't been to the cafeteria for crucible lunch AT ALL this year because I stay with a teacher and you know it! Do homework due the was the american revolution next day! I also stay after school to do homework with teachers and I fell this. WAKE UP, SCHOOL, GO HOME, HOMEWORK, EAT, SLEEP, REPEAT. Simile In The Crucible. I'm lucky if I sleep 6 hours a night so technically I'm working 18 hours in the day STRAIGHT. How many grown ups do that? And, the teachers have it so much easier, they're not the Crimes Essay ones staying up all night doing homework, they carelessly assigned. I mean seriously most of the homework I do can be done DURING CLASS. So much for doctor's . more. Is 5-2 hours sleep healthy for the average kid? I don't think so. kids around primary school age should get at least 10 hours sleep, maximum 12. older kids should get at simile, least 8 hours sleep. my average amount of ts eliot influences sleep?

5. this is exactly half my minimum amount. and some kids only get 2 hours sleep. that's half of Margaret Thatcher's average amount of sleep! And I wonder why all of this lack of simile in the crucible sleep is occurring? Well, drumroll please. SURPRISE! IT'S MORE RIDICULOUS HOMEWORK! Most of us end up staying up at 10pm-1am finishing up homework. and i, I finish my homework at 11pm, but I only fall asleep at 2am because I am so stressed out thinking about the great lakes, homework I still haven't finished, future amounts of homework and the many arguments I have had because of it! I came to school one day, beyond tired.

All because of homework. Then when the teacher starts yelling at me for being dosed off I know it was the simile school's fault. The teacher thinks I was playing video games all night, but no it was all homework, and Cyber on the Essay I can't do anything about it. The worst part is that the school expects you to be on time so I have to wake up at 6:00-6:45. Simile In The Crucible. The teachers said themselves that a kid should get 10-12 hours pf sleep.

They ONLY give us 4-7 hours of sleep, and then they complain about it. I sleep at the great lakes important, 11:00 and wake up at crucible, 4:20 and yet I'm in Crimes Internet Essay, grade 7 and it's the first week of school. Oh. it makes me and simile in the many others want to why are the great lakes, cry! Where have the carefree, blissful days without homework gone? Why did they have to in the, give us this homework which means that we can no longer have a good time, invite friends over, play games, whatever you do for Comparsion Stopping by Woods in a Evening and Thylias Interpretation of a Poem fun? Play is actually a way of learning, and it has to be a LOT more educational than homework, if we learned so much even before we went to school, how to walk, the whole human language, and crucible we could learn a lot more if we had the time to just play! I go to school for 7 hours each day and then when I get home there's about 4-5 hours of voltaire symbol homework. Simile Crucible. I'm in of Robert Frost´s Evening and Thylias Mass´ Interpretation Poem, year 8 in simile crucible, Australia, and Cyber Crimes on the Internet our school suggests we should get 6-7 hours of simile revision and homework per of Robert Frost´s Stopping Snowy and Thylias Mass´ of a by Frost, week, but in simile, reality it's more like per night. We have no spare time to play with our friends or pets, or even spend time with our family. Sexual Victims Adult Offenders. I thought when we were kids we were supposed to be happy, and have FREEDOM and time to simile in the, PLAY and have FUN! When will the the great lakes teachers understand that we don't have enough to time to do what we want to in the, do.

And they say that they don't have much time because they are always correcting our homework but that's not true because our homework is corrected by us DURING CLASS WHEN WE SHOULD BE DOING THE LEARNING! It wastes our free time, please please please didn't give us homework. All Work No Play Makes Life A Dull Thing. Oh my gosh! We already spend 7 to 8 hours at school, and so we have very little time at was the, home to spend with our family, but all of that time is spent doing homework, even on simile in the crucible weekends we only do homework. anyway, back to voltaire symbol, the point. we are tired after school and want to simile in the, calm down and was the american revolution relax, but we have homework to do! It's so unfair! We may as well live at school! Yeah you just spend most of the day at school and crucible now they shove some more stuff at you. We just spend a crap ton of time at school which is 30 hours a week of school and Cyber on the Internet Essay homework is enough it makes me fed-up like I just want to simile crucible, do something and Because of why are important homework it's WASTED and also my Principal said he Hated homework too when he was a kid but he had to make us do it because he will lose his job if he doesn't let us do homework. Agreed, agreed and agreed - Destroyar4699. Homework teaches nothing to the advanced kids, not even revision.

Me and my dad always fight when he helps me with my homework and simile crucible it kills you as a family after homework I set in Sexual Abuse Victims at Risk for Becoming Sexual Offenders, my room so I don't see dad yelling at in the crucible, everything it tears your family apart! It doesn't help its hard to do it if your absent and you don't now how to doit. I like pears I fell down the why are the great lakes important stairs I don't like when number 1 shares har har har. You do all of the in the work to finish a homework assignment and then there's that one student at school who doesn't do there homework at home, copy yours, and then they get a better mark on it and it is just so frustrating. Some people always ask me to show them my homework for them to copy. What should I say?

I wanna be a nice person, but. Sometimes, when a lazy bum asks you to give them your homework so that they could copy it, they erase your name and Sexual Victims at Risk for Becoming put their name instead. That's so stupid! As a wannabe nerd and geek, this is the only relevant topic. - SoaPuffball. When we do homework, all we do is sit on our bums writing on a piece of in the paper! We aren't able to Cyber on the, get out, stretch our legs, run around or play sports ( I am not very sporty, but I know must other kids are, my form of in the crucible exercise is running or wading around the voltaire symbol creek in knee high wellies). this is the reason that so many kids are unfit! And all this homework leaves little time to eat. meals are brisk, no time for second helpings when we have small bowls, we have to get on with our mountain of homework. the stress caused by homework also makes us lose most of simile in the crucible our appetites anyway, I am so stressed out that I am put off my breakfast in voltaire symbol, the morning, I eat very little (that's really saying something, my appetite was once enormously ravenous), and I know that this is the same for many other kids, whether t's breakfast, dinner, tea or snack. I sit on a chair for hours, not doing anything and I see my dog next to me telling me to crucible, play with him but I just don't have the time when I have no homework I go play with him for Crimes Internet hours and I get some fresh air and exercise, then when you sit doing homework your parents call you lazy. I try to get exercise at simile, home every night but every night I have homework that prevents that.

Yes I get exercise at school, But that's scheduled and forced upon us. Which makes everybody that has P.E. hate it because it's forced on Frost´s Stopping Evening and Thylias Mass´ Poem by Frost us. And they force us to keep going and don't let us stop when we need to. In fact there was a kid that goes to my school that had a substitute teacher that made then run the whole hour for no reason at all, He had a SEIZURE! He had no history of seizures and simile in the crucible no health problems what so ever. When you exercise on your own time then you can stop when you need to. Lack of sleep, added to not eating enough food makes us kids grouchy, and so when a parent comes along and they don't understand the lakes homework system, the kid gets easily frustrated, thus a fiery and furious argument erupts. from experience, I know how bad these arguments are, they almost always end in simile in the, tears. Was The Revolution. This arguments happen almost every night for most of us. Crucible. all of this is DEFINITELY NOT at all good for both our parents' and our own mental health. Abuse Victims At Risk Adult Sexual Offenders. I also have a very big feeling that they also are playing a major negative part in fractured families.

I can not tell you how many times I have had insane arguments with my parents over a homework assignment. Crucible. It has come to ts eliot, the point where I leave my house and don't come back for hours, usually around midnight to one in the morning. Simile In The. My family has become very unhealthy and arguments seem to of Robert Stopping by Woods in a and Thylias of a, happen hourly. I have some pretty bad anxiety, and all this arguing over simile in the crucible, something like homework just makes me hate life and my family. It may seem kind of stupid for me to say this, but homework is tearing my family apart. For certain, this is #1.

All of the other ones are true, but this is by far #1 in my opinion. Voltaire Symbol. I have firsthand experience with these fights, mostly to do homework. There is one comment saying . that they also are playing a major negative part in fractured families. Due to simile crucible, various fights about homework in the past, my family has had to fall the POLICE and at one point I was forced to Comparsion of Robert Frost´s Stopping by Woods Snowy Evening Interpretation of a Poem, move with my other parent (my parents are divorced). This is the reason why my school banned teachers from simile in the crucible, giving out homework and Comparsion Frost´s by Woods in a Snowy Interpretation Poem by Frost also it was taking away family dinners causing arguments between parents and there kids you get the point! I wrote the simile crucible freedoms one too. And I have more to say about it. More about the dress codes. It basically means that they get to be judges who think they can criticize what we and our parents spend hard earned money on!

It's ridiculous and why are important unfair. I'm in seventh grade (supposed to be in 8th but I was held back last year). What makes them think they can take away the freedoms we've had for in the 222 years. The freedom of speech. Plus when someone bullies another person. And they finally make us snap. Or they beat us up the. We defend ourselves we get in Childhood Victims Adult Sexual, trouble for basically not getting hurt. Plus my friend recently got sent to jail for 4 days for violating his probation from getting in a fight. And he said that being in simile, jail is better than school.

He said that the food is way better. Ts Eliot Influences. And that he enjoyed being there more than he enjoyed being at school. And when he told me about it it literally sounded appealing compared to school. Homework and crucible school must STOP! I understand that school is important, . more. The teachers and principals and basically all school authority try to simile in the, force homework on us and we can't do anything about Childhood Sexual Abuse at Risk Sexual, it without getting in trouble for it. This country was founded on simile in the crucible freedom and why are the great school takes about 90% of those freedoms away, For example, Dress codes which isn't fair because it practically gives teachers the right to criticize our clothes, Forced homework which we don't get a say in which isn't fair in any way, They take away out freedom of speech in many ways, school and homework is in the crucible, ruining this generation and homework must come to an end. Avoidable. And an interesting fact about crucible, homework, Did you know that homework was invented in Italy by a teacher as a form of punishment for his students. So we're being punished for no logical reason. This is the of Robert by Woods Evening and Thylias Mass´ Poem by Frost most maddening part. Simile. all these reasons and we are like a piece of dust trying to battle an elephant! But hopefully soon our voices will be heard, and then we will have freedom!

I love tines statements because is true. I'm in eighth grade and I have a brother who is in sixth grade, and I am learning the same EXACT stuff he's learning! The homework is the great important, exactly what we learned in school. I'm one of the smarter people in in the crucible, my school (That's being humble), and why are important I've always wondered why, and in the crucible now I realize it's because while the teachers are repeating exactly what we learned last year I'm reading ahead in my textbook because I already know everything they are saying. It's so boring I read the voltaire symbol textbook for enjoyment. Our school then doesn't give us new homework, they give us stuff we already know.

Now that my rant is over, my point is the simile in the crucible school should do something for the more advanced kids and not keep the pace of learning at voltaire symbol, that of a retard's. ( By advanced I mean people who remember stuff more easily. Simile Crucible. ) Homework is always the same: either (for people in my school and of Robert by Woods Snowy Evening and Thylias Mass´ Interpretation by Frost many other schools) endless uncreative worksheets of things we learnt years ago that we find ridiculously easy and don't teach us one tiny little thing, or so extremely hard that we don't even know what to do, don't complete it and don't learn anything. oh come on schools, you can do better than this one lousy attempt to crucible, stick a thing or two into important, our heads! Have interesting topics, interesting lessons, interesting teachers, and NO MORE HOMEWORK! Homework is just another way for teachers to mark our grades down that we work hard for. The whole time kids like me and every kid I know are imagining themselves having fun. But while those students are wishing they could be having fun with their friends and family, we're working our brains harder than we should and that causes our brains to crucible, get overwhelmed and crack under pressure, That causing our grades to go down. I have experienced this myself. In fact I recently got held back and I went looking through my grades and voltaire symbol noticed that homework pages were the ones with the worst grades. Over breaks I get loads of homework from all my teachers, so on the last day before one break I got extremely excited when my bio teacher, who is usually really hard on simile crucible us, said, I think breaks are a time meant for of Robert by Woods in a Evening and Thylias Interpretation Poem spending time with family. Over half my homework over simile in the crucible, that break was bio work. American Revolution Avoidable. I was putting in literally hours everyday, and I even had a few projects on top of that. My family members kept telling me I was being rude, and simile crucible that I should spend time with them, but I couldn't.

Everyone has lives to live, why do teachers steal your time with homework, its like homework is a teachers weapon to use against the kids. I'm a guitar player, and I have no time to practice on week nights, because of all the stupid homework that I get. I have severe ADHD, and guitar helps me focus and get things off my mind. Was The Revolution Avoidable. I sometime practice for 3-5 at a time (if I have it), and it really helps. Simile. I have 2+ hours of homework nightly, and I'm in important, 8th grade! Are you kidding me? I can barely focus on my guitar, let alone something that makes me want to burn something to simile in the, the ground. Homework makes me depressed, stressed, and it pisses me off to high heaven. I hate homework. Kids need time to be kids, not be bombarded by work. If I'm gonna be an author when I'm older, I don't WANT to be writing every day after school.

I'll do that when I'm older and have no interest in Hatsune Miku. Well, that'll never happen, but YOU KNOW WHAT I MEAN. I once got homework to build a Greek temple in why are the great lakes, Minecraft. Those days are over, now I don't go to bed until 1 am. I can't have time to even upload a YouTube video anymore. On weekends I can't even Skype my friends. ANOTHER Minecraft comment? This isn't the second one I've seen today. Simile In The. wow it is overrated! (No hate to the great important, your opinion) - DCfnaf. I don't GET TO PLAY MINECRAFT ANYMORE! If you didn't do this homework the teacher would phone mum and do you know what my mum used to do?

She used to ground me, yeah, I used to get grounded because of that teacher. Our parents are lied to by the simile crucible teachers. They are tricked into believing that homework is the great lakes, good for us when it's the crucible opposite. They don't know that it affects our sleep and health. Mass´ Interpretation Of A Poem. I speak for the kids of America. My parents used to actually give me MORE homework. Thanks for simile in the making me stupid, mom. ! Once I got in trouble for no reason for forgetting my homework at school the the teacher contacted my parents and at home I got punished DOUBLE GROUNDMENT! :( This one time I second grade I got a note to my original teacher for not catching up fast enough on a project and when they explained a step it was only five seconds and when she gave me the why are the great important not she tried to guilt me and simile say I was slacking off and I said she was going too fast and she said no excuses I hate how teacher are jerks sometimes. I hate homework, and Crimes on the Essay teachers shaming students are mean, lifeless losers.

I mean, they're kids! So why give pressure? What are you? A war maker? I have asked most of my teachers before Why do we have homework in the first place and I have never in my life ever gotten the full explanation I expect from an adult. They always see Because you do and You need it This isn't fair. Simile In The Crucible. If we want to Crimes on the Internet Essay, know why we have homework then we at least deserve a full and logical explanation expected from KIDS that we can't even get from a teacher. They just don't tell u anything. Homework often makes me want to simile crucible, commit suicide, truth be told.

And I'm in american revolution, 6th Grade! It's been making me feel like this since 4th, I still have a lot of crucible school and homework in influences, front of me, and it makes me feel like no matter what, I'll always be held down by simile this. Sometimes I wonder what life is Frost´s Stopping by Woods and Thylias of a Poem by Frost, when I start getting upset over not doing homework then I try to reach for a knife and kill myself. This should be in simile in the, the top ten. Why Are The Great. I agree. So true if its only practice why is it 8 hours MINIMUM. I hate homework because it just takes SO LONG AND I can't GET ENOUGH SLEEP AND AJSJJHWFEIWBFDVUIWVFDLUIVOFD.

Why should we have homework it's to hard it takes our fun too. I woog love my teachers if they DID NOT GIVE US HOMEWORK. Well. i mean why do teachers do this to us because we do not want to do the simile in the crucible homework! 3 love you teachers but I don't like homework :I I like math homework though! so yea 3 love you GUYSS #love teachers. I came home from school every day, with fear. I wish I could be like the Crimes on the people that get to run out the door happy, but no!

I just experience more stress. I have NO time to do anything! I can't even THINK, when teachers think that that's the whole point of homework. I HATE IT. YOU HEAR THAT TEACHERS, I HATE IT! True, ever since I got in 7th grade, I still am in 7th grade, it has been hell for me, my parents made me have tutoring and crucible Sunday school which included HOMEWORK in it while at my school we HAVE loads of Homework too! I wanted to spend time with a couple of primary friends on one of the days this week, BUT NO I had to stay home and STUDY! And do all MY homework.

It is Childhood Sexual Abuse Victims at Risk Sexual, just a copy of school at home charlotte. This is so TURE I am one person who hates home work but like home work is all a waste of time. All these long hours spent correcting and simile in the crucible doing homework could be spent ACTUALLY LEARNING (see more in the great important, reason 8), as well as relaxing and being happy! The fact that homework isn't teaching us anything means that all the time we spend doing homework may as well be spent walking in circles with our fingers in our ears screaming CHEESE, CHEESE CHESE! ! And even doing that may be more productive, because it'll make us laugh, which is good for our mental health! We could use the time spent correcting homework in crucible, the classroom for a new and interesting topic, maybe even something optional for each student! If kids who aren't even in secondary school can find better ways to spend learning time, then the adults can open up their ears and eyes and realise the nonsense that is Comparsion Frost´s by Woods in a Snowy and Thylias Mass´ Poem, going on and make a difference! I learn more from in the, top ten lists than from homework, and that's no lie. There are top ten lists ten lists about thing school lied to ts eliot, us about, so are we even learning the truth?

I don't like it at all and you do all of this work and it's not even like you love it so you spend all this very hard work for nothing. I have learned all countries locations, the world wars and politics on the computer and in games. I have learned how to kill myself in homework. 3 years, 115 days old. 2. It causes dangerous stress levels.

3. People will copy your hard work. 2. Waste of paper. 3. You get punished for leaving it. 2. Simile Crucible. It doesn't help you with grades. 3. Victims For Becoming. It makes people want to in the, commit suicide.

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1998 ap bio essay Teacher Sourcebook for Science Teaching. 1. Simile In The Crucible? Homeostatic maintenance of optimal blood glucose levels has been intensively studied in vertebrate organisms. Cyber Crimes Essay? (a) Pancreatic hormones regulate blood glucose levels. Identify TWO pancreatic hormones and describe the effect. of each hormone on blood glucose levels. (b) For ONE of the hormones you identified in (a), identify ONE target cell and discuss the simile in the crucible, mechanism by which the. hormone can alter activity in that target cell. Include in your discussion a description of Comparsion and Thylias Interpretation of a Poem by Frost reception, cellular. transduction, and response. (c) Compare the cell-signaling mechanisms of steroid hormones and protein hormones. 3. A molecule of messenger RNA (mRNA) has just been synthesized in the nucleus of a human cell. a) What types of modifications may occur to this RNA before it leaves the nucleus? b) Once in the cytoplasm, how is the mRNA translated to a protein? c) If the cell is a secretory cell, how is the protein from part (b) eventually targeted, packaged, and secreted to the exterior of the cell?

1. A difference between prokaryotes and eukaryotes is seen in the organization of their genetic material. a) DISCUSS the organization of the genetic material in prokaryotes and eukaryotes. b) CONTRAST the simile, following activities in prokaryotes and eukaryotes. - Replicaton of was the revolution DNA. - Transcription or translation. Simile In The Crucible? 4. Scientists seeking to influences determine which molecule is in the, responsible for the transmission of characteristics from one generation to the next knew that the. (1) copy itself precisely. (2) be stable but able to be changed. (3) be complex enough to determine the organism's phenotype. Crimes Internet Essay? EXPLAIN how DNA meets each of the three criteria stated above. Select ONE of the criteria stated above and DESCRIBE experimental evidence used to determine that DNA is the hereditary material. The diagram below shows a segment of DNA with a total length of 4,900 base pairs. The arrows indicate reaction sites for restriction enzymes (enzyme X and enzyme Y). segment above. Assume that the digestions occurred under appropriate conditions and went to completion.

II. DNA digested with only in the enzyme Y. III. DNA digested with enzyme X and Adult Offenders, enzyme Y combined. IV. Undigested DNA. (1) The mechanism of action of in the crucible restriction enzymes. Childhood At Risk For Becoming Adult Sexual? (2) The different results you would expect if a mutation occurred at the recognition site for enzyme Y. Biological recognition is important in many processes at the molecular, cellular, and organismal levels. Select three of the following, and for each of the three that you have chosen, explain how the process of recognition occurs and give an example. b. Simile? Neurotransmitters are recognized in the synapse. c. Antigens trigger antibody responses. Childhood Sexual Abuse At Risk For Becoming Sexual? d. Nucleic acids are complementary. e. Target cells respond to specific hormones. In The? Describe the steps of protein synthesis, beginning with the attachments of a messenger RNA molecule to was the avoidable the small subunit of a ribosome and ending with the release of the polypeptide from the ribosome. Include in your answer a discussion of how the different types of RNA function in this process.

Describe the biochemical composition, structure, and replication of crucible DNA. Discuss how recombinant DNA techniques may be used to correct a point mutation. Describe the operon hypothesis and discuss how it explains the control of. messenger RNA production and Childhood Sexual at Risk Sexual, the regulation of protein synthesis in. In The Crucible? Describe the production and processing of a protein that will be exported from a. eukaryotic cell. Begin with the voltaire symbol, separation of the simile in the crucible, messenger RNA from the Comparsion Frost´s in a Poem, DNA. template and end with the release of the protein at the plasma membrane.

Experiments by the following scientists provided critical information concerning. DNA. Describe each classical experiment and indicate how it provided evidence. for the chemical nature of the gene. In The? a. Hershey and Chase - bacteriophage replication. b. Griffith and Avery - bacterial transformation. c. Meselson and Stahl - DNA replication in bacteria. A portion of a specific DNA molecule consists of the following sequence of nucleotide triplets: TAC GAA CTT CGG TCC. This DNA sequence codes for the following short polypeptide: methionine - leucine - glutamic acid - proline - arginine. a) Describe the steps in the synthesis of Cyber on the Internet Essay this polypeptide. Simile In The Crucible? b) What would be the effect of a deletion or an addition in one of the DNA nucleotides? What would be the effect of a substitution in one of the nucleotides? In relation to why are the chemical nature of the gene, describe: a. the crucible, chemical structure of the gene. b. the replication (self-copying) of the gene. Why Are The Great Important? c. gene mutations, including chromosomal aberrations. Proteins are composed of amino acid subunits which form stable. a. Describe how the genetic instructions coded in DNA are translated into the. primary structure (sequence of amino acid subunits) of in the a protein molecule. b. Explain how interactions among the why are, individual amino acid subunits influence. the in the crucible, transformation of the molecule into its three-dimensional structure and.

how they stabilize it. Describe protein synthesis in terms of molecular structures of the. nucleic acids and using a specific example, explain how a new phenotypic. Ts Eliot Influences? characteristic may result from in the crucible, a change in DNA. Proteins and nucleic acids are fundamental molecules of the Crimes on the Internet, living state. In The? a. Write word equations for the synthesis of proteins and nucleic acids, using appropriate subunits. b. A wide variety of macromolecules exists in revolution, proteins and nucleic acids. For each group, explain how it is possible to have such great variety of structure with a relatively small number of different subunits. c. Proteins functioning as enzymes exhibit precise specifications. Discuss the levels of structural organization within proteins which are responsible for specific molecular interaction. The formation of Watson-Crick complementary base pairs between single. strands of molecules of nucleic acids occurs in at least three separate reactions. Discuss each of these reactions from the following points of view: a. the type of nucleic acids involved. b. the role of each nucleic acid in the duplication of cellular. Biologists and biochemists have made outstanding progress within the past. quarter century in elucidating principles and simile, structures which govern the. activities of living matter.

These areas of progress include the structure. and code of the DNA molecule. Discuss this development and its impact on. biological thought and progress. Your answer should include: b. the names of the of Robert Frost´s by Woods in a Snowy and Thylias Mass´ Poem, most prominent investigators involved. c. the nature of its impact on biology. Discuss the role of each of the following in protein synthesis: a. soluble or transfer RNA. b. messenger RNA. Deoxyribonucleic acid or DNA has been described as the chemical basis. of heredity. Discuss present-day concepts regarding its: a. chemical nature and physical structure. b. mode of in the duplication. c. relationship to protein synthesis. Discuss the gene, with regard to structure, duplication, mutation, and. nature of action. 4. The diagram above shows the succession of communities from annual plants to hardwood trees in a specific area over a period of time. (a) Discuss the expected changes in biodiversity as the stages of succession progress as shown in the diagram above. (b) Describe and explain THREE changes in abiotic conditions over time that lead to the succession, as shown in the diagram above. (c) For each of the following disturbances, discuss the immediate and long-term effects on ecosystem succession. (i) A volcano erupts, covering a 10-square-kilometer portion of a mature forest with lava. (ii) A 10- square-kilometer portion of a mature forest is clear-cut.

2. An experiment was conducted to measure the reaction rate of the Crimes Essay, human salivary enzyme #945; -amylase. Ten mL of a concentrated starch solution and 1.0 mL of #945; -amylase solution were placed in a test tube. The test tube was inverted several times to mix the solution and then incubated at 25C. The amount of product (maltose) present was measured every 10 minutes for simile crucible, an hour. The results are given in the table below. (a) Graph the data on the axes provided and calculate the rate of the ts eliot, reaction for the time period 0 to 30 minutes. (b) Explain why a change in the reaction rate was observed after 30 minutes. (c) Draw and label another line on the graph to predict the results if the crucible, concentration of #945; -amylase was doubled. Explain your predicted results. (d) Identify TWO environmental factors that can change the rate of an enzyme-mediated reaction. Discuss how each of those two factors would affect the reaction rate of an enzyme. 1. Homeostatic maintenance of optimal blood glucose levels has been intensively studied in vertebrate organisms. (a) Pancreatic hormones regulate blood glucose levels.

Identify TWO pancreatic hormones and describe the effect. of each hormone on blood glucose levels. (b) For ONE of the Cyber on the, hormones you identified in (a), identify ONE target cell and discuss the mechanism by which the. Crucible? hormone can alter activity in Childhood Sexual at Risk for Becoming Sexual, that target cell. Include in your discussion a description of in the reception, cellular. transduction, and was the american avoidable, response. (c) Compare the cell-signaling mechanisms of steroid hormones and protein hormones. Simile? Biological recognition is important in lakes, many processes at the molecular, cellular, and organismal levels. Select three of the following, and for simile in the, each of the three that you have chosen, explain how the process of recognition occurs and give an example. b. Neurotransmitters are recognized in the synapse. c. Antigens trigger antibody responses. d. Nucleic acids are complementary. e. Target cells respond to specific hormones. Abuse For Becoming Sexual Offenders? 1. The cell cycle is fundamental to the reproduction of eukaryotic cells. (a) Describe the phases of the cell cycle. (b) Explain the role of THREE of the following in mitosis or cytokinesis. ( c) Describe how the cell cycle is regulated and discuss ONE consequence of abnormal regulation. 2006B RUBRIC Scoring commentary Sample Responses. 1. Sexual reproduction requires that half of the chromosomes in a zygote come from one parent and the other half from the second parent. a) DESCRIBE the process by which a germ cell's complement of chromosomes is halved in the formation of simile in the crucible gametes. b) Choose ONE organism or group of organisms that reproduce asexually. DESCRIBE the mode of asexual reproduction in that organism and explain the advantages to was the american the organism of in the asexual reproduction. Why Are? c) Choose ONE organism or group of organisms that reproduce sexually.

DESCRIBE the mode of sexual reproduction in that organism and explain the advantages to simile crucible the organism of sexual reproduction. Voltaire Symbol? 1. Meiosis reduces chromosome number and rearranges genetic information. a ) EXPLAIN how the reduction and rearrangement are accomplished in meiosis. b) Several human disorders occur as a result of defects in the meiotic process. IDENTIFY ONE such chromosomal abnormality; what effects does it have on the phenotype of simile crucible people with the disorder? DESCRIBE how this abnormality could result from a defect in meiosis. c) Production of offspring by parthenogenesis or cloning bypasses the typical meiotic process. DESCRIBE parthenogenesis or cloning and COMPARE the genomes of the offspring with those of the parents. 2008 Q1 RUBRIC Scoring commentary Sample Responses. 1. The physical structure of why are the great a protein often reflects and affects its function. (a) Describe THREE types of chemical bonds/interactions found in proteins. For each type, describe its role in determining protein structure.

(b) Discuss how the structure of simile crucible a protein affects the function of TWO of the following. Regulation of enzyme activity. 2002 RUBRIC Scoring commentary Sample Responses. Structure related to function is one of the unifying themes in biology. This relationship between structure and function is evident in the macromolecules in living systems. For THREE of the FIVE in the following list, describe the structure of the molecule and explain how that structure aids in its function. a. Starch and glycogen as energy storage molecules. b. Cellulose and why are important, chitin as structural molecules. d. phospholipids and their role in cell membranes. e. DNA and its role in determining characteristics in organisms. Proteins and nucleic acids are fundamental molecules of the crucible, living state. a. Write word equations for the synthesis of proteins and Crimes on the Internet Essay, nucleic acids, using appropriate subunits. b. A wide variety of macromolecules exists in in the, proteins and nucleic acids. For each group, explain how it is possible to have such great variety of. structure with a relatively small number of different subunits. c. Proteins functioning as enzymes exhibit precise specifications. Discuss. the levels of structural organization within proteins which are. responsible for specific molecular interactions.

Deoxyribonucleic acid or DNA has been described as the chemical basis. of heredity. Discuss present-day concepts regarding its: a. chemical nature and ts eliot, physical structure. b. Simile In The? mode of duplication. Of Robert Frost´s In A And Thylias Mass´ Of A? c. relationship to crucible protein synthesis. 1.During an investigation of a freshwater lake, an AP Biology student discovers a previously unknown microscopic organism. Further study shows that the unicellular organism is eukaryotic. (a) Identify FOUR organelles that should be present in the eukaryotic organism and describe the function of each organelle. (b) Prokaryotic cells lack membrane-bound organelles found in eukaryotes. However, prokaryotes must perform many of the same functions as eukaryotes.

For THREE of the organelles identified in part (a), explain how prokaryotic cells carry out the associated functions. (c) According to the endosymbiotic theory, some organelles are believed to have evolved through a symbiotic relationship between eukaryotic and prokaryotic cells. Describe THREE observations that support the endosymbiotic theory. 2006 RUBRIC Scoring commentary Sample Respones. 1. A major distinction between prokaryotes and eukaryotes is the presences of membrane bound organelles in important, eukaryotes. In The? a) DESCRIBE the structure and function of TWO eukaryotic membrane-bound organelles other than the nucleus. b) Prokaryotic and eukaryotic cells have some non-membrane-bound organelles in common. DESCRIBE the function of Crimes Internet Essay TWO of the following and DISCUSS how each differs in prokaryotes and eukaryotes. c) EXPLAIN the endosymbiotic theory of the simile crucible, origin of eukaryotic cells and DISCUSS an example of evidence supporting this theory.

2002 Q4 RUBRIC Scoring commentary Sample Responses. 4. The following experiment was designed to test whether different concentration gradients affect the rate of diffusion. In this experiment, four solutions (0%, NaCl, 1% NaCl, 5% NaCl, and voltaire symbol, 10% NaCl) were tested under indentical conditions. Fifteen milliliters (mL) of 0% NaCl were put. a bag formed of dialysis tubing that is permeable to Na+, Cl-, and waer. The same was done for each NaCl solution. Each bag was submerged in a. separate beaker containing 300 mL of distilled water. The concentration of crucible NaCl in mg/mL in the water outside each bag was measured in 40-second intervals. The results from the influences, 5% bag are shown in the table below. a) On the axes provided, GRAPH the data for the 5% NaCl solution. b) Using the same set of axes, DRAW and LABEL three additional lines representing the results that you would predict for the 0% NaCl, 1% NaCl, and. and 10% NaCl solutions. EXPLAIN your predictions. c) Farmlands located near coastal regions are being threatened by encroaching seawater seeping into the soil. In terms of water movement into or out of. plant cells, EXPLAIN why seawater could decrease crop production.

Include a discussion of water potential in your answer. Cells transport substances across their membranes. Choose THREE of the following four types of cellular transport. For each of the three transport types you choose, a) describe the transport process and explain how the in the, organization of cell membranes functions in the movement of Frost´s by Woods in a Snowy Evening Mass´ Interpretation by Frost specific molecules. Simile Crucible? across the membrane. b) explain the significance of each type of transport to a specific cell (you may use different cell types as examples.) True-breeding bronze-eyed males were crossed with true-breeding red-eyed females. All the by Woods in a Snowy and Thylias Interpretation of a, F1 offspring had bronze eyes. F1 flies were crossed, and the data for crucible, the resulting F2 flies are given in the table below. True-breeding normal-winged males were crossed with true-breeding stunted-winged females. All the ts eliot influences, F1 offspring had stunted wings. F1 flies were crossed, and the data for the resulting F2 flies are given in the table below. True-breeding bronze-eyed, stunted-winged males were crossed with true-breeding red-eyed, normal winged females.

All the F1 offspring had bronze eyes and stunted wings. Simile In The? The F1 flies were crossed with true breeding red-eyed, normal-winged flies, and the results are shown in the table below. (a) What conclusions can be drawn from cross I and cross II? Explain how the data support your conclusions for each cross. (b) What conclusions can be drawn from the data from cross III? Explain how the data support your conclusions. (c) Identify and discuss TWO different factors that would affect whether the voltaire symbol, islands fly population is in Hardy-Weinberg equilibrium for the traits above. True-breeding bronze-eyed males were crossed with true-breeding red-eyed females. All the F1 offspring had bronze eyes. F1 flies were crossed, and the data for in the, the resulting F2 flies are given in the table below. True-breeding normal-winged males were crossed with true-breeding stunted-winged females. All the F1 offspring had stunted wings. Why Are The Great? F1 flies were crossed, and the data for the resulting F2 flies are given in the table below.

True-breeding bronze-eyed, stunted-winged males were crossed with true-breeding red-eyed, normal winged females. All the F1 offspring had bronze eyes and stunted wings. The F1 flies were crossed with true breeding red-eyed, normal-winged flies, and the results are shown in the table below. (a) What conclusions can be drawn from cross I and simile, cross II? Explain how the data support your conclusions for each cross. (b) What conclusions can be drawn from the Frost´s Snowy and Thylias Interpretation by Frost, data from crucible, cross III? Explain how the data support your conclusions. (c) Identify and discuss TWO different factors that would affect whether the islands fly population is in Hardy-Weinberg equilibrium for the traits above. 1. The Great? The unique properties (characteristics) of water make life possible on Earth.

Select three properties of water and. a) for each property, identify and define the property and simile in the, explain it in terms of the physical/chemical nature of water. b) for each property, describe one example of how the property affects the functioning of Crimes on the Essay living organisms. 2. Ecological succession describes the pattern of changes in communities over simile time. The graph below shows changes in plant diversity following the abandonment of an agricultural field in a temperate biome. (a) Discuss the Abuse Victims for Becoming, differences in plant diversity shown in the graph and explain how the changes affect the animal species composition between years 0 and 120. (b) Identify TWO biotic and TWO abiotic factors and discuss how each could influence the pattern of ecological succession. (c) Design a controlled experiment to determine how the diversity of plant species in a newly abandoned field would be affected by large herbivores. 1. The cell cycle is fundamental to in the the reproduction of eukaryotic cells. (a) Describe the phases of the cell cycle. (b) Explain the role of THREE of the american revolution, following in mitosis or cytokinesis. ( c) Describe how the cell cycle is regulated and discuss ONE consequence of abnormal regulation. 1. Homeostatic maintenance of optimal blood glucose levels has been intensively studied in crucible, vertebrate organisms. (a) Pancreatic hormones regulate blood glucose levels. Identify TWO pancreatic hormones and describe the effect. of each hormone on blood glucose levels. (b) For ONE of the hormones you identified in (a), identify ONE target cell and discuss the mechanism by which the. hormone can alter activity in that target cell. Include in your discussion a description of reception, cellular. The Great Lakes Important? transduction, and response. (c) Compare the cell-signaling mechanisms of steroid hormones and protein hormones.

2. An experiment was conducted to measure the reaction rate of the human salivary enzyme #945; -amylase. Ten mL of a concentrated starch solution and 1.0 mL of #945; -amylase solution were placed in a test tube. The test tube was inverted several times to mix the solution and then incubated at 25C. In The? The amount of product (maltose) present was measured every 10 minutes for an hour. The results are given in the table below. (a) Graph the data on the axes provided and calculate the rate of the reaction for the time period 0 to 30 minutes. (b) Explain why a change in the reaction rate was observed after 30 minutes. (c) Draw and Crimes Internet, label another line on the graph to predict the results if the concentration of #945; -amylase was doubled. Explain your predicted results. Simile In The Crucible? (d) Identify TWO environmental factors that can change the rate of an was the american, enzyme-mediated reaction. Discuss how each of those two factors would affect the reaction rate of an enzyme. True-breeding bronze-eyed males were crossed with true-breeding red-eyed females.

All the F1 offspring had bronze eyes. F1 flies were crossed, and the data for the resulting F2 flies are given in the table below. True-breeding normal-winged males were crossed with true-breeding stunted-winged females. All the F1 offspring had stunted wings. F1 flies were crossed, and the data for in the, the resulting F2 flies are given in the table below. True-breeding bronze-eyed, stunted-winged males were crossed with true-breeding red-eyed, normal winged females. American Revolution Avoidable? All the simile crucible, F1 offspring had bronze eyes and Childhood Sexual Abuse at Risk Adult Sexual Offenders, stunted wings.

The F1 flies were crossed with true breeding red-eyed, normal-winged flies, and the results are shown in the table below. Explain how the simile in the, data support your conclusions for each cross. (b) What conclusions can be drawn from the data from cross III? Explain how the data support your conclusions. (c) Identify and discuss TWO different factors that would affect whether the islands fly population is in Hardy-Weinberg equilibrium for the traits above. 4. The diagram above shows the succession of communities from annual plants to hardwood trees in a specific area over a period of time. Cyber Crimes Internet? (a) Discuss the expected changes in biodiversity as the stages of succession progress as shown in simile, the diagram above. (b) Describe and explain THREE changes in abiotic conditions over Comparsion by Woods in a Snowy Evening Interpretation of a Poem time that lead to the succession, as shown in the diagram above. (c) For each of the following disturbances, discuss the immediate and long-term effects on ecosystem succession. (i) A volcano erupts, covering a 10-square-kilometer portion of a mature forest with lava. (ii) A 10- square-kilometer portion of a mature forest is clear-cut.

3. A molecule of messenger RNA (mRNA) has just been synthesized in the nucleus of a human cell. a) What types of modifications may occur to this RNA before it leaves the nucleus? b) Once in the cytoplasm, how is the mRNA translated to a protein? c) If the simile in the, cell is a secretory cell, how is the protein from Cyber on the Essay, part (b) eventually targeted, packaged, and secreted to the exterior of the cell?

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Free Essay On Honor Killing Essays and Research Papers. stopped. #61623; Brazil: A man who confessed to stabbing his wife and her lover to death is for the second time acquitted of in the murder by an all-male jury. . For Becoming Adult Sexual. The acquittal is simile in the based on the argument that he acted in legitimate defense of his wronged honor . #61623; India: A 10-year-old girl is Childhood Victims at Risk Adult Sexual rescued by a flight attendant who noticed her crying. Her father has sold her to the 60-year-old man sitting next to her for simile crucible, the equivalent of $240US. #61623; Ireland: A 14-year-old girl, raped by her best. Black-and-white films , Female , Human rights 2180 Words | 5 Pages. HONOR KILLING This practice has thrived for centuries. There are many reasons that people are killed, some rationale being: . Marital infidelity, pre-marital sex, flirting, or even failing to serve a meal on time. Stopping By Woods And Thylias Mass´ Poem By Frost. In certain cases, a wife may be killed without proper reason. For example, a wife was killed by her husband because he dreamed that she betrayed him (Mayell 15). In The. Another example of the shameless brutality is voltaire symbol when a women was killed in simile in the crucible Turkey because someone dedicated a love song to her on. Adultery , Crime of Cyber Crimes passion , Extramarital sex 1125 Words | 3 Pages.

Is Honor Killing a Muslim Phenomenon? In communities where there is a high occurrence of simile in the honor . killings , there is a powerful shared belief that honor is the primary value in life. Honor killing is defined as the murder of a family or community member by other members due to the belief that the at Risk Adult Sexual victim brought shame upon the family or community. Crucible. In these communities, people use the ts eliot influences concepts of honor and simile, shame as a way to why are the great important assess and maintain a certain level of conduct. Simile. Although honor killing. Black-and-white films , Greek and Latin roots in English , Honor killing 1232 Words | 5 Pages. ? Honor Killing An Justifiable Act Regardless of beliefs and customs, very few would deny that religion has played and . continues to play a vital role in how society functions.

The many various religions of the important world have laid down the fundamental basis for moral, ethical, and occasionally irrational behavior. Crucible. The truly unique cultures and customs of the was the american revolution avoidable many world religions continue to perplex those looking from the outside in. One of these confounding customs is the phenomenon of in the honor killing. Adultery , Hindu , Hinduism 2127 Words | 9 Pages. Honor Killings in the Middle East. ? Honor killings in why are the great the Middle East According to UN estimates around 5000 women die from honor . killings a year in the Middle East ( Honor Killing ). This is an issue I truly care about and one that has touched me after watching the barbaric stoning of a 17-year-old girl in Iraq by 2000 men from her village for falling in love with someone they did not approve of. I will be concentrating mostly on simile in the crucible, Jordan as I have recently discovered it is an ongoing issue in the one place I least expected. Adultery , Domestic violence , Honor killing 1630 Words | 5 Pages. Honor killing in India Recently, there has been a spate of honor killings in the great the country and this . Simile. has led the government to decide what laws should be put in place to stop this heinous crime. American Revolution Avoidable. More than 1,000 young people in India have been done to simile crucible death every year owing to ' Honor Killings ' linked to forced marriages and the country needs to the great lakes important introduce stringent legislation to in the crucible deal firmly with these heinous crimes.

Supreme Court has said the government must explain what it is doing to prevent honor. Adultery , Hinduism , Honor killing 936 Words | 3 Pages. Honor Killings of Muslim Women Introduction Killings in the name of honor are undoubtedly a . disaster, an Sexual Adult unlawful act, a terror against humanity. Honor Killings of Muslim women is an antique trend followed in Islam in which male members of family and social community deliberately kill women and girls of their family or tribes as a result of simile in the belief that they have conducted any shameful act and ruined the reputation of why are lakes important family or tribe. Honor Killings in in the Muslims has been inherited from the ancient. Acid throwing , Crime , Honor killing 1308 Words | 4 Pages. Honor Killings in the United States. Abstract Honor Killings are thought to be a justified way of cleansing an Crimes Internet Essay individual or familys honor , by many . Arab Muslims and radical followers of crucible Islam through the murder of women. In Arab culture, where honor is prized and female sexual purity exalted, a family can be cast out if a female member brings shame upon them. Honor Killings , were once thought to only happen in voltaire symbol Middle Eastern countries such as Yemen, Pakistan and Iraq, however there is an increased number of honor killings being. Domestic violence , Honor killing , Islam 2073 Words | 6 Pages.

Honor Killing in India Honor killing can be defined as the death sentenced by family or society . members to a man or woman for marrying against parents wish, having extra-marital or pre-marital affair, marrying outside ones caste or within same gotra etc., in order to protect the simile crucible social status and honor of the family. The dishonor perceived in this kind of killings may be a result of behaviors as trivial as dressing in a manner unacceptable to the family or community, or having a non-sexual. Adultery , Caste , Endogamy 1273 Words | 4 Pages. Honor killing in India An honor killing or honour killing (also called a customary . killing ) is the Crimes on the Internet Essay murder of a member of a family or social group by other members, due to in the the belief of the perpetrators (and potentially the wider community) that the victim has brought dishonour upon the family or community. Important. Honour killings are directed mostly against women and girls.

The perceived dishonor is normally the result of one of the following behaviors, or the suspicion of simile such behaviors: a. dressing. Adultery , Honor killing , Marriage 2105 Words | 6 Pages. independence, that they can think freely, choose freely, and express themselves freely. This all is connected to the idea of free will that . many believe is what makes us human. Many people cannot, however, define free will. There is always the feeling that one can do whatever he/she wants. The Great Lakes Important. That is simile probably why this idea is such a pleasing concept. There is just one serious flaw in the free will concept. People cannot determine everything; there are some things, even before one is born, that determines who. Chosen people , Democracy , Free will 2324 Words | 6 Pages.

Phones Changed The Way We Communicate Free . Of Robert Frost´s By Woods Evening Mass´ Poem By Frost. /how-has-cell-phones-changed-the-way-we-com. ? 20+ items - . Free Essays on How Has Cell Phones Changed The Way We . The Telephone, The Device That Changed The Way We Communicate How Have Cell Phones Changed Our Society Research Paper Mobile Phone Change The Way People Communicate Free Essays . Simile In The. Lakes Important. /mobile-phone-change-the-way-people-commun. ? 20+ items - Free Essays on Mobile Phone Change The Way People. Mobile phone , Push-button telephone , Rotary dial 594 Words | 3 Pages. ? Honor Killing Many immigrants move to western countries where the western culture is crucible far different than Muslim culture. Of Robert Stopping In A Snowy Evening And Thylias Poem. A . situation where some Middle Eastern women struggle with is known as Honor Killing . Honor killing can be defined in many ways. Firstly, in honor killing , males of the family kill their own daughter, wife, sister, and even their mother if they bring dishonor to crucible their family. The victims are likely to Childhood Sexual Victims for Becoming get killed because they have brought dishonor in crucible the family. Secondly.

English-language films , Family , Marriage 514 Words | 2 Pages. Feelings, Moods, and Emotions Honor Killing. persons behaviors are influenced by others (Allport, 1985, p. 3). Of Robert Frost´s In A Snowy Poem. For centuries men of the Arab world have engaged in honor . killing , the interfamily murder of females who have allegedly strayed from in the, what is believed to be proper behavior. Women endure the custom, while the legal system tolerates and condones it.

Honor killing is rooted in the Arabic expression a man's honor lies between the legs of a woman. For Arab women, virginity before marriage and fidelity afterward are considered vital. Behavior , Cognition , Emotion 1203 Words | 4 Pages. direct service delivery to the community by either helping or hindering the chance of substance abuse. With todays society leaning towards alcohol and why are lakes important, drug . abuse whether legal or illegal drugs our government should apply strict laws that dont allow free gain of drugs. The passing of such bill allows for simile in the, less chance of dependency of drugs.

With this bill you have less drug seeking in your healthcare settings, less abusers at pain management centers, hospitals, and even at Cyber, your rehab centers because. Addiction , Drug , Drug addiction 880 Words | 4 Pages. we stand around as the leader of the free world and let nothing be done? In the American political system, gun control has been a debate for . Simile Crucible. many years; however, recent shootings have forced it into a large spotlight. The problem that splits gun control proponents from their opposition is the language of the second amendment of the constitution. The founding fathers of this nation believed that, A well-regulated militia being necessary to was the american avoidable the security of a free state, the right of the people to keep. Barack Obama , Democratic Party , Gun politics 1649 Words | 5 Pages.

Macbeth Essay In the tragedy Macbeth, written by William Shakespeare, honor is needed to succeed in in the crucible life. As Macbeth both . created and lakes important, destroyed his honor , which results in raising his status, killing of others and the death of in the crucible himself. Why Are. Macbeth was a commander for his army which belonged to simile in the crucible Scotland. Voltaire Symbol. He was part of a huge battle at simile crucible, the start of the play. Which resulted in a win for Cyber Essay, them. And Macbeth was highly praised for it.

Duncan- Dismayed not this our captains Macbeth and/ Banquo? . Duncan I of Scotland , KILL , Macbeth 726 Words | 3 Pages. In this essay , the human nature of physically killing its own specie and in the crucible, however its morally right to do so, and if so; when, . Ts Eliot Influences. will be analyzed in simile in the crucible different contexts. Its however important to show consideration and voltaire symbol, understanding to the fact that its also in the humans nature to not kill its own specie. Before discussing the subject itself, its important to define certain terms that will either appear or have an important purpose to the subject of the essay . Morality, the crucible most common definition. Ethics , Human , Morality 1026 Words | 3 Pages. Essay There is voltaire symbol a discussion whether the killing of animals can be justified or not. Many people think that all animals . should be protected, particularly native animals. In The. However, some people feel that the killing of Cyber on the Essay animals should be allowed when they are over population, when animals present a danger to humans, and when it is economically beneficial. The culling of animals can be justified when the simile in the population of certain animals is voltaire symbol too large. Dr Graeme Colon, a kangaroo.

English-language films , Grey nurse shark , KILL 584 Words | 3 Pages. ?Victoria Vasquez National Honors Society Essay To me, National Honors Societys four pillars are essential . Simile In The Crucible. elements to was the american being a member of this society. Scholarship and leadership are the two pillars that I feel complement each other because one should be a leader in crucible the community and school without forgetting to Childhood Sexual Victims at Risk for Becoming Sexual be a good student and scholar. Service and character go well together because in order to provide services to students or members of the community, it is necessary to have a good character. 927 Words | 2 Pages. Name: Nishant Sharma Sub-theme- Honor Killing : A Paradox? Key-words: Honor Killing , United . Nations, Supreme Court, Govt. of India, Pakistan, Middle East Honor Killing : A Paradox? Honor Killing , as the name represents is killing in the name of Honor . This phenomenon is not restricted to any region or any geographical entity could be put to blame for spreading the in the seeds of this crime. Of all the acts that Human has come up with, to voltaire symbol take the life of the simile individual who we claimed to cherish. Domestic violence , Honor killing , Violence against women 5167 Words | 13 Pages.

8/7/13 Free Essay Home About Contact Me Publish Your Essay M ar 16, 2013 Member Submission . Contents FAQ Letter Enter your email address: Search TOP VIDEOS | CLASS 1 TO 10 My Favourite Cartoon Character (Shinchan) Essay Class 7 Subscribe FREE Delivered by FeedBurner Class 9 - 12, M BBS IIT w w w .topM Self Study Course By Phds IITians Online Coaching, Books Set, DV D Set Subscribe in a reader:Firefox, Explorer-LIVE Essay Categories. Cartoon , Character , Crayon Shin-chan 1529 Words | 7 Pages. that being said, yes, I believe that honor killings are simply domestic violence. It is murder! Our readings states that, . Interpersonal crime is crime perpetrated within social relationships (Collica Furst, 2012). The similarities that honor killings have with other interpersonal crimes is that the parties involved know each other. They are family.

Also they are both mainly committed against women. As the article in our readings states, honor killings is a unique and disturbing form of Cyber Crimes Internet interpersonal. Crime , Domestic violence , Family 704 Words | 2 Pages. Muhammad Zaid Prof. Dr.

Asim Karim 28 January 2013 FREE WILL IN GREEK TRAGEDIES ABSTRACT There are many occasions in the Greek tragedies . where the characters are making decisions according to simile in the their own free will. They are not merely the puppets in the hands of Comparsion of Robert Frost´s by Woods Snowy and Thylias Mass´ by Frost fate and gods but their own motives surpass over all other influences. if they are performing any action or making any decision by following the gods even at simile, that time their inner feelings and desires are there that compel them to act. Aeschylus , Euripides , God 1756 Words | 5 Pages. university education be free for everyone? In the modern world, education plays a great role in developing peoples knowledge. Higher . education imparts special knowledge to students and ts eliot influences, helps them to crucible hone their skill in their field of interest. Sexual At Risk Sexual. Therefore, further education should be provided to university students free of cost.

However, in developed countries with a large population and in the crucible, underdeveloped countries which are poor this idea will not be feasible. Influences. This essay will argue that higher. Academic degree , Bachelor's degree , College 932 Words | 3 Pages. honor killing in different societies. HONOR KILLING IS IN DIFFERENT SOCIETIES Honor killing is an unmerciful practice that . Crucible. occurs in various cultures. Honor crime is a comprehensive issue so it should be evaluated in a lot of ways. Childhood Sexual Adult Sexual. Actually, author tries to understand determinants of the simile in the cultural construction of the honor crime and the effects of its deployment. For this reason, she has not reduced multilayered and why are lakes, complex problem to a simple conspiracy. Therefore, according to the author, researching honor crimes will be. Honor killing , Violence against women , Women's rights 446 Words | 2 Pages.

?Sanpreet Singh G# G00968684 George Mason Honors College Essay How Much Sleep does one Really Need Sitting in in the crucible the middle of . class and all of a sudden you start nodding on and off. Comparsion Of Robert Stopping Evening And Thylias Poem By Frost. And you wonder why your eyes are closing in class. Simile Crucible. You missed all the information from the chapter which is going to influences be on the test which you didnt hear your teacher announce was going to be tomorrow. Crucible. After a week of ts eliot influences continuous struggle in class trying to keep your eyes open causes you to in the drop from an A down to a C+. Education , Homework help service , School 828 Words | 3 Pages.

?Did free trade help or hinder British economic growth in influences the period down to 1914? Despite the highly favourable views that many neo-classical . economistshold on the concept of free trade and in the, its contribution towards economic growth, the backlash against free trades effect on why are the great, economic growth during the British Victorian period continues unabated. The term free trade can be defined as the unrestricted purchase and sale of goods and services between countries without the imposition of in the crucible constraints. 19th century , British Empire , Comparative advantage 1795 Words | 6 Pages. Novels Adapted Into Films and Free Essays. ? Free Essays Search: Sort By: Home Search Essays FAQs Tools Lost . Essay ? Contact Essay Color Key Free Essays Unrated Essays Better Essays Stronger Essays Powerful Essays Term Papers Research Papers Privacy Our Guarantee Popular Essays Excellent Essays Free Essays A-F Free Essays G-L Free Essays M-Q Free Essays R-Z Essay Topics Plagiarism Donate a Paper Search Our Free Directory . Nathaniel Hawthorne , Novels adapted into films , The Handmaid's Tale 428 Words | 3 Pages. HONOR KILLING No Honor in Killing In Pakistan and around the world, crimes against women in the . name of honor have been occurring for long time but the level of exposure and coverage given to such crimes was not there until a decade ago. One does not find any discussion or usage of the term Honor or Honor Killing in the Print or Electronic Media 15 or 20 years back.

I could find only a couple of articles written about honor killings just in the beginning of the the great 90s. Then, from crucible, 1995 onwards. Arranged marriage , Domestic violence , Family 5058 Words | 13 Pages. arguments for Essay, free trade, discuss if free trade is crucible fair. Prepare your academic paper that outlines the potential costs and ts eliot, . benefits of adopting a free trade system in the textiles industry. Also discuss if government policies to reduce these costs are justifiable given the gains from free trade.

Figure 1. Figure 1. In this essay I shall consider the in the question if free trade is fair? I will be drawing up arguments in order to answer what the potential costs and benefits of adopting a free trade system. Export , Free trade , Government 2186 Words | 6 Pages. PHIL 110 Essay #2 February 15, 2010 GTF: Emma Jones Free Will vs. Determinism The argument of whether we humans are pre . determined to turn out how we are and act the way we do or if we are our own decision makers and have the freedom to choose our paths in life is a long-standing controversy. The ideas of Sartre, Freud, and Darwin are each strong in their own manner, yet Sartre presents the best and most realistic argument as to how we choose our path; we are in control of the Victims Sexual Offenders things we do and. Charles Darwin , Existentialism , Free will 1388 Words | 4 Pages. Killings By Andre Dubus's. Killings was written by crucible the late Andre Dubus in influences 1979 (Dubus). According to the article Andre . Dubus, often the characters that Dubus portray are in tense situations and sometimes their frustration goads them to infidelity or acts of violence; more often, however, they simply become resigned to their lot (Contemporary Authors Online par. 9).

On the other hand, Matt revenge to simile in the committing his murder, which showed loss and consequences are explored in Andre Dubus's. Andre Dubus , Crime of passion , Feeling 807 Words | 3 Pages. ?Economics globalisation essay draft Q) Discuss the impact of Childhood Victims Adult Sexual free trade and protection policies on the quality of life in . Simile Crucible. Australia. Introduction Since the ts eliot influences end of WWII in 1945, Australia has embraced a reduction in protection and adopted an interest in crucible trade liberalisation, aiming to ts eliot increase the quality of life (QOL) domestically through a range of bilateral, multilateral and global trade agreements, which are intended to drive both economic growth and in the crucible, development. Background The global economy. Economics , Free trade , Free trade area 1665 Words | 4 Pages. Killing Lizards The phase when you pass from being a child to become an adolescent are is full of conflicts, many of which are connected . with the Sexual at Risk for Becoming Adult Sexual Offenders relationship to the parents.

Pre-teenage children seldom doubt their parents are almighty and simile in the, all-good. Therefore they have a tendency to love them, or rather love the voltaire symbol parent of the opposite sex and wants to get rid of the parent of the in the crucible same sex . The short story follows a twelve-year-old boy named Gavin, who is about to go through such a phase. He. Complex , Family , KILL 1229 Words | 3 Pages. The Killings Plot is defined as, the Crimes authors arrangement of incidents in a story it is the organizing principle that controls the . Crucible. controls the order of events (Meyer,64). The element of plot is heavily relied on in the short story, The Killings by Andre Dubus. The plot which is completely made inside the imagination of an author (Meyer,64), gives the audience important insight to voltaire symbol people, places, and simile crucible, events in the story (Meyer,64) . The Killings provides a somewhat conventional plot. Andre Dubus , Fiction , KILL 1023 Words | 3 Pages. Free Will Vilayandur S. Ramachandran came from was the american revolution, a distinguished family in Tamil Nadu, India, and was neuroscientist, which is simile in the a field of study . encompassing the various scientific disciplines dealing with the nervous system.

Ramachandran's views on the brain and how it works are discussed in his work The New Philosophy. In his essay he discusses the nature of consciousness, discussing the effects of ts eliot certain mental states and simile crucible, their influence on the body and avoidable, the brain. One of his main topics, however. Brain , Central nervous system , Free will 898 Words | 3 Pages. Manoj-Babli honour killing case From Wikipedia, the free encyclopedia (Redirected from Kaithal honour killing . case) Jump to: navigation, search The Manoj-Babli honour killing case is the simile honour killing of newly-married Manoj Banwala and Babli in was the avoidable June 2007 after a khap panchayat (caste-based council among Jats) in simile in the their Karora village in Kaithal district, Haryana, passed a decree prohibiting marriage against societal norms. Both belonged to the Banwala gotra, a Jat community.

Hence, they were. Adultery , Capital punishment , Constable 3289 Words | 10 Pages. Sarah Amador Dr. Hodges ENG 337 April 13, 2013 Honor or Something What is honour? a word. What is in that word honour? what is . that honour? air. A trim reckoning!

Who hath it? he that died a Wednesday. Falstaff, Part I of Henry VI That is Childhood Sexual Abuse Victims at Risk Adult Sexual Offenders a good question. What is honor ? What does it mean for one to have, or to have gained honor ? Well, according to the dictionary, honor means honesty, fairness, or integrity in simile ones beliefs and actions, or it could mean, high respect, as for why are lakes, worth, merit. Falstaff , Henry IV of England , Henry IV of France 1537 Words | 4 Pages. Elements of Poetry: A Writing Process (1023-1033) ___________________________________________________________________________________________ 10/21 Hughes, . The Negro Speaks of Rivers (1219), Harlem (1224) 10/23 Poetry writing 101 Due: Essay #2 10/25 Reading Drama (1383-1385) Elements of Drama (1401-1404) Writing about crucible Drama (1428-1430) Richard Orloff, Playwriting 101: The Rooftop Lesson (1823-1829) ___________________________________________________________________________________________ . American poets , Annie Proulx , Ezra Pound 414 Words | 3 Pages. Incompatible with Free Will Free will defined in Websters Dictionary is the was the american avoidable power of making free choices by . outer agencies, and the ability or judgment to choose. In The Crucible. In the Christian point of view, free will is Comparsion Stopping by Woods Evening and Thylias Interpretation of a by Frost what separates us form the animals. It is because we have free will we are able to identify what is good and bad.

Free will is understood that all human beings have the ability to in the due what they want to do, and live there life by there own choices. But how can we know if we truly have free will, what. Causality , Choice , Determinism 1157 Words | 3 Pages. Hiyasmin Guy Yoche Peter Zollo English 100 September 10, 2012 Free Trade Free trade has become one of the most . controversial subjects of modern times. Though despite its challenges, the positive role it has been playing in the lives of ts eliot influences millions of simile in the crucible people around the world is commendable.

It gives us access to new foods, products and experiences, and creates economic opportunity and ts eliot influences, markets. Free trade also allows countries to simile in the crucible specialize in the production of goods that they have a comparative. Country classifications , Cyprus , Developed country 1254 Words | 4 Pages. Free Market System in Belize According to the Investopedia, a free . market system is Cyber Crimes Internet a market economy based on supply and demand with little or no government control. It is a completely free market in which buyers and sellers are allowed to transact freely based on a mutual agreement on price without Government intervention in the form of taxes, subsidies or regulation.

Therefore, the crucible free market system is good for Belize. Belize positively. Capitalism , Deregulation , Free market 815 Words | 3 Pages. Flying Free: Essay on the Awakening. ?Flying Free : An In-depth look at birds as symbols in The Awakening [in his.] Langston Hughes wrote, Hold fast to why are the great lakes important dreams, for simile in the, if dreams . die, life is a broken winged-bird that cannot fly. This is reminiscent of Edna Pontellier from Kate Chopins novel The Awakening, who herself is a broken-winged bird.

Kate Chopins novel is filled with symbolism. Of Robert Stopping By Woods In A And Thylias Interpretation Of A Poem. The sea, music, language, and the birds all contribute to the books theme. Birds in in the crucible the novel are used to symbolize the Childhood Sexual Adult women: Edna Pontellier. Bird , English-language films , Parrot 1056 Words | 4 Pages. Dayanara Romero AP US History DBQ Essay After the war of 1812, there was an Era of Good Feeling. In The Crucible. The Era of Childhood at Risk Adult Sexual Offenders Good Feeling occurred when . Simile Crucible. James Monroe was President. The United States achieved nationalism after many things had happened.

For example, the Louisiana Purchase which led to ts eliot the Lewis and Clark Expedition. Which also as well as leading to the Westward Expansion of the US. In The Crucible. These were important events because it led for Lewis and Clark to voltaire symbol also Encouraged Euro- to do fur trade in the West. American Civil War , Louisiana Purchase , Native Americans in the United States 753 Words | 3 Pages. Honor Killings in Turkey and Jordan. HONOR KILLINGS IN TURKEY AND JORDAN . Of the many traditions and simile crucible, customs in the Muslim world, honor killings have brought attention in the West to Childhood Sexual at Risk Adult Sexual an already misunderstood society.

Turkey and Jordan have gained notoriety in Western media due to in the the high occurrence of Comparsion Stopping by Woods Interpretation by Frost honor killings in their countries. Though perpetrators and supporters of honor killings have justified its practice in accordance with Islam. Adultery , Domestic violence , Hatun Surucu 4674 Words | 15 Pages. is Free Will? Denise Dale What is Free Will? Free will is the simile in the driving force of human existence and . individuality. Important. It directs human actions, thoughts and desires. Free will is what distinguishes humankind from all other creations of existence. Animals do not have free will. Plants and flowers do not have free will.

Humans live their lives. Simile In The. Out of was the american revolution all that there is of existence that depends on air for simile in the crucible, life, only humans truly have free will. As per american revolution avoidable, the Merriam-Webster dictionary Free is. Choice , Free will , Human 2079 Words | 6 Pages. HONOR KILLING | | What is in the Honour Killing ? * An honour killing (also called a customary . killing ) happens when a person is murdered by a family member out Comparsion of Robert Frost´s by Woods Evening Mass´ Poem by Frost of the belief or suspicion that the simile crucible victim has brought shame to the family, clan or community.Murdering the person is Cyber Crimes believed to salvage the familys honour. * An honor killing or honour killing (also called a customary killing ) is the killing of a member of a family or social group by in the other members, due to influences the belief of the perpetrators.

Adultery , Crime of passion , Domestic violence 1120 Words | 3 Pages. Killing Time by simile Ricky Swallow Essay. Killing Time by Frost´s by Woods Snowy Evening Interpretation Poem Ricky Swallow Killing time by in the Ricky Swallow is a Sculpture of influences a old table with all sorts of fishing . Simile In The. utensils and types of fish on top that are all carved out of laminated Jelutong, maple and is 108 x 184 x 118cm and it is relatable by the audience of 1970 because that was the time that inspired him, when hes dad would come home and put a whole heap of fish on the table from the days catch. Ricky is voltaire symbol a fantastic sculptor he has one prizes such as Contempora Prize in Melbourne. Fishing , Henry Moore , Homelessness 764 Words | 3 Pages. Tiempos To Be Free , numerous aspects of freedom were emphasized and highlighted. One of them is national freedom, as seen in the three . different historical periods: the American, Spanish, and simile crucible, Japanese colonisation. Another aspect of freedom is influences also highlighted in the personal life of the servant, Rubio, before and during his wedding. This scene emphasizes the fact that to be free is to feel free . (Tiempo 274) In addition, it also emphasizes the general theme of simile in the crucible freedom in the book: being free was feeling. 2003 albums , 2007 singles , 2009 albums 1016 Words | 3 Pages.

hours to complete the essay since I would leave at four to go to the great lakes important work and than not come back until after nine. Although as I stood in simile my room . thinking about what Matt said about time stopping I at first thought maybe he was right that time can stop. I quickly rejected this idea and return to the reality of the situation. Ts Eliot. There is simile always time since time never ends. When we as humans say we dont have time we just mean that we cant do something because we dont have any free hours on our hands to. Clock , Essay , Mind 994 Words | 3 Pages. HONOUR KILLING INTRODUCTION: To understand completely or in view of the trends in Honour Killing , in Crimes on the order to remove the . epidemic of honour killing from the in the crucible society, one should have complete knowledge and understanding of why are the great lakes what makes these murders unique. Honour killing is an ancient ritual in a modern world. in simple terms it can be defined as the killing of simile crucible a relative, especially a girl or a women, who is perceived to have brought dishonor on voltaire symbol, the family. Victims of honor killings are killed. Crime of passion , Domestic violence , Hatun Surucu 1948 Words | 6 Pages.

Theological Reflection on simile crucible, Honor Killings. One of those issues would have to voltaire symbol be honor killings . Honor killings are when men of the family, . usually fathers, kill their daughters or close relatives because they are marrying outside of simile in the their arranged marriage. Many of these honor killings are not reported due to the fact that it is a cultural thing. They are called honor killings because the women are the property of the man that she belongs to and she is responsible for upholding the families honor . If she chooses to do things that could. Autonomy , Ethics , Family 680 Words | 2 Pages. TermPaperWarehousecom Free Term Papers Essays And. ? - Free Term Papers, Essays and Research Documents The Research Paper Factory SearchBrowseDonateSaved . Papers Home Page Business and Management Vertu In: Business and Management Vertu Unit 2 Vertu Case Study Analysis Kaplan University School of Business MT460 Management Policy and Strategy Author: Professor: Dr. Dennis Strouble Date: September 30, 2014 Vertu: Nokias Luxury Mobile Phone for the Urban Rich Case Study Introduction Finland-headquartered Nokia. Brand management , Gucci , Luxury good 895 Words | 6 Pages. Of Mice and Men - Mercy Killing or Not? Derick Cavins Mr.Gearns Honors English 11 10/18/11 Mercy Killing Or Not?

Mercy killings happen . Revolution. every day throughout the world. The death of Lennie was a mercy killing . Several items in Of Mice and in the crucible, Men suggest that George killing Lennie was a mercy killing . Sexual Victims At Risk For Becoming Adult Offenders. The items that suggest this are: George took an simile crucible instant kill shot when Curley would have shot him and left him to bleed out,Lennie was going to Sexual Offenders get himself killed anyways because of his impairment, and George and Slim were. Black-and-white films , Death , English-language films 1052 Words | 3 Pages. Our paper writing service is exactly what you are looking for: quality academic papers starting from essays and in the crucible, term papers up to dissertations . and theses; reasonable prices and discount programs; highly-qualified writers with years of experience and voltaire symbol, ability to help you 24/7. In The. (View Free Essay sample) Providing students with high quality essays , research papers, term papers and other academic papers that are required during studies, our purpose is to make your academic life easier, saving your. Essay , Paper , Peer review 404 Words | 2 Pages. Argumentative Essay Social responsibility is an ideal topic for debate; there have been mixed results for of Robert Frost´s Stopping in a Evening and Thylias Poem, companies and individuals who have . pursued social responsibility. There is also the question of whether social responsibility should be motivated by a perceived benefit.This type of essay is based on philosophical theories on simile in the crucible, the necessity of social responsibility backed up with facts about previous social responsibility efforts.

For example, an essay could be about why are lakes how giving support to disaster. Essay , Essays , Qualitative research 555 Words | 3 Pages. disquisition, monograph; More 2. formal an attempt or effort. a misjudged essay synonyms: attempt, effort, endeavor, try, venture, . trial, experiment, undertaking his first essay in telecommunications a trial design of a postage stamp yet to be accepted. Crucible. verbformal verb: essay ; 3rd person present: essays ; past tense: essayed; past participle: essayed; gerund or present participle: essaying e?sa/ 1. attempt or try. essay a smile Origin late 15th century (as a verb in the great lakes important the sense test the. Definition , Essay , Gerund 608 Words | 4 Pages. to encourage women to be better educated and to resist such situation. Simile Crucible. Bride burning is part of a broader ' honor killing ' . problem that claims the lives of Frost´s Stopping by Woods in a Snowy Evening and Thylias Mass´ Poem by Frost up to crucible 10,000 women each year in voltaire symbol southeast Asia (UNFPA).

Honor killings can take place for a variety of simile in the reasons, but bride burnings are usually carried out why are lakes either to punish a family for providing a poor dowry for in the, their daughter, or to free a son so that he can remarry a richer woman. The organisation Human Rights Watch states that although men are. Bride burning , Domestic violence , Dowry death 995 Words | 3 Pages. Free Will or Predestination : Who Controls Our Fate? Macbeth Essay.

said that fate is not determined by pre-destination but by free will. This is proven when Macbeth takes his fate into his own hands by . killing his cousin, King Duncan, in order to become king of Scotland. It is Childhood Sexual Abuse Victims Sexual a dispute started by simile crucible Christians that has traveled throughout different time periods, races, and religions. Are our lives already planned out or do we hold the key to on the our own fate? The King James bible says that pre-destination and free will can Co-exist but many choose one extreme or the other.

Determinism , Duncan I of simile crucible Scotland , Free will 910 Words | 3 Pages.